Cell sensitivity assays: The MTT assay

J. van Meerloo; G.J.L. Kaspers; J. Cloos

doi:https://doi.org/10.1007/978-1-61779-080-5_20

Cell sensitivity assays: The MTT assay

J. van Meerloo, G.J.L. Kaspers, J. Cloos

Research output: Contribution to journal › Article › Academic

1639 Citations (Scopus)

Abstract

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro cytotoxic effects of drugs on cell lines or primary patient cells. In this chapter the protocol of the assay is described including important considerations relevant for each step of the assay as well as its limitations and possible applications.

Original language	English
Pages (from-to)	237-245
Number of pages	9
Journal	Methods in Molecular Biology
Volume	731
DOIs	https://doi.org/10.1007/978-1-61779-080-5_20 https://doi.org/10.1007/978-1-61779-80-5_20
Publication status	Published - 2011

Keywords

3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide
Cytotoxicity assay
Drug sensitivity assay
MTT
Viability assay

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title = "Cell sensitivity assays: The MTT assay",

abstract = "The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro cytotoxic effects of drugs on cell lines or primary patient cells. In this chapter the protocol of the assay is described including important considerations relevant for each step of the assay as well as its limitations and possible applications.",

keywords = "3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, Cytotoxicity assay, Drug sensitivity assay, MTT, Viability assay",

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JO - Methods in Molecular Biology

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Cell sensitivity assays: The MTT assay

Abstract

Keywords

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