Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines

N. Castro Kreder; R. ten Cate; H.M. Rodermond; C. van Bree; N.A.P. Franken; M.Z. Zdzienicka; J. Haveman

doi:https://doi.org/10.1269/jrr.45.385

Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines

N. Castro Kreder, R. ten Cate, H.M. Rodermond, C. van Bree, N.A.P. Franken, M.Z. Zdzienicka, J. Haveman

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect

Original language	Undefined/Unknown
Pages (from-to)	385-391
Journal	Journal of Radiation Research
Volume	45
Issue number	3
DOIs	https://doi.org/10.1269/jrr.45.385
Publication status	Published - 2004

Keywords

AMC wi-eigen

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https://doi.org/10.1269/jrr.45.385

https://pure.uva.nl/ws/files/3619794/45083_203599y.pdf

Cite this

@article{cfb6321008924d80a1194248a9a7a408,

title = "Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines",

abstract = "The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect",

keywords = "AMC wi-eigen",

author = "{Castro Kreder}, N. and {ten Cate}, R. and H.M. Rodermond and {van Bree}, C. and N.A.P. Franken and M.Z. Zdzienicka and J. Haveman",

year = "2004",

doi = "https://doi.org/10.1269/jrr.45.385",

language = "Undefined/Unknown",

volume = "45",

pages = "385--391",

journal = "Journal of Radiation Research",

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TY - JOUR

T1 - Cellular Response to Pulsed Low-dose Rate Irradiation in X-ray Sensitive Hamster Mutant Cell Lines

AU - Castro Kreder, N.

AU - ten Cate, R.

AU - Rodermond, H.M.

AU - van Bree, C.

AU - Franken, N.A.P.

AU - Zdzienicka, M.Z.

AU - Haveman, J.

PY - 2004

Y1 - 2004

N2 - The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect

AB - The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect

KW - AMC wi-eigen

U2 - https://doi.org/10.1269/jrr.45.385

DO - https://doi.org/10.1269/jrr.45.385

M3 - Article

C2 - 15613783

SN - 0449-3060

VL - 45

SP - 385

EP - 391

JO - Journal of Radiation Research

JF - Journal of Radiation Research

IS - 3

ER -