TY - JOUR
T1 - Cerebral microdialysis of interleukin (IL)-1beta and IL-6: extraction efficiency and production in the acute phase after severe traumatic brain injury in rats
T2 - extraction efficiency and production in the acute phase after severe traumatic brain injury in rats
AU - Folkersma, Hedy
AU - Brevé, John J. P.
AU - Tilders, Fred J. H.
AU - Cherian, Leela
AU - Robertson, Claudia S.
AU - Vandertop, W. Peter
PY - 2008/12
Y1 - 2008/12
N2 - BACKGROUND: As a research tool, cerebral microdialysis might be a useful technique in monitoring the release of cytokines into the extracellular fluid (ECF) following traumatic brain injury (TBI). We established extraction efficiency of Interleukin(IL)-1ss and Interleukin(IL)-6 by an in vitro microdialysis-perfusion system, followed by in vivo determination of the temporal profile of extracellular fluid cytokines after severe TBI in rats. MATERIALS AND METHODS: In vitro experiments using a polyether sulfon (PES) microdialysis probe especially developed for recovery of macromolecules such as cytokines, were carried out to establish the extraction efficiency of IL-1ss and IL-6 from artificial cerebrospinal fluid (CSF) with defined IL-1ss and IL-6 concentrations. In vivo experiments in which rats were subjected to TBI or sham and microdialysis samples were collected from the parietal lobe for measurement of cytokines. FINDINGS: The extraction efficiency was maximal 6.05% (range, 5.97-6.13%) at 0.5 microl/min(-1) and decreased at higher flow rates. Both cytokines were detectable in the dialysates. Highest IL-1ss levels were found within 200 min, highest IL-6 concentrations were detected at later intervals (200-400 min). No differences were found between the TBI and control groups. CONCLUSIONS: Cerebral microdialysis allows measurement of cytokine secretion in the ECF of brain tissue in rats
AB - BACKGROUND: As a research tool, cerebral microdialysis might be a useful technique in monitoring the release of cytokines into the extracellular fluid (ECF) following traumatic brain injury (TBI). We established extraction efficiency of Interleukin(IL)-1ss and Interleukin(IL)-6 by an in vitro microdialysis-perfusion system, followed by in vivo determination of the temporal profile of extracellular fluid cytokines after severe TBI in rats. MATERIALS AND METHODS: In vitro experiments using a polyether sulfon (PES) microdialysis probe especially developed for recovery of macromolecules such as cytokines, were carried out to establish the extraction efficiency of IL-1ss and IL-6 from artificial cerebrospinal fluid (CSF) with defined IL-1ss and IL-6 concentrations. In vivo experiments in which rats were subjected to TBI or sham and microdialysis samples were collected from the parietal lobe for measurement of cytokines. FINDINGS: The extraction efficiency was maximal 6.05% (range, 5.97-6.13%) at 0.5 microl/min(-1) and decreased at higher flow rates. Both cytokines were detectable in the dialysates. Highest IL-1ss levels were found within 200 min, highest IL-6 concentrations were detected at later intervals (200-400 min). No differences were found between the TBI and control groups. CONCLUSIONS: Cerebral microdialysis allows measurement of cytokine secretion in the ECF of brain tissue in rats
KW - Acute Disease
KW - Animals
KW - Brain Injuries/immunology
KW - Brain/immunology
KW - Disease Models, Animal
KW - Enzyme-Linked Immunosorbent Assay/methods
KW - Extracellular Fluid/chemistry
KW - Interleukin-1beta/analysis
KW - Interleukin-6/analysis
KW - Male
KW - Microdialysis/methods
KW - Neurochemistry/methods
KW - Rats
KW - Rats, Sprague-Dawley
KW - Time Factors
U2 - https://doi.org/10.1007/s00701-008-0151-y
DO - https://doi.org/10.1007/s00701-008-0151-y
M3 - Article
C2 - 19031041
SN - 0001-6268
VL - 150
SP - 1277-84; discussion 1284
JO - Acta neurochirurgica
JF - Acta neurochirurgica
IS - 12
ER -