TY - JOUR
T1 - Changes in vascular density in resected tissue of 97 patients with mild malformation of cortical development, focal cortical dysplasia or TSC-related cortical tubers
AU - Veersema, Tim J.
AU - de Neef, Andrew
AU - van Scheppingen, Jackelien
AU - Ferrier, Cyrille H.
AU - van Eijsden, Pieter
AU - Gosselaar, Peter H.
AU - van Rijen, Peter C.
AU - Spliet, Wim G. M.
AU - Braun, Kees P. J.
AU - Mühlebner, Angelika
AU - Aronica, Eleonora
PY - 2019
Y1 - 2019
N2 - Recent studies suggested a possible association between malformations of cortical development and microvascular density. In this study we aimed to further elucidate the relation between microvascular density and cortical developmental abnormalities in a cohort of 97 patients with epilepsy and histologically proven mild malformation of cortical development (mMCD), focal cortical dysplasia (FCD) or tuberous sclerosis complex (TSC). Surgical tissue samples were analyzed with quantitative measures of vessel density, T-cell response, microglial activation and myelin content. Subsequently, the results were compared to an age- and localization matched control group. We observed an increase in microvasculature in white matter of TSC cortical tubers, which is linked to inflammatory response. No increase was seen in mMCD or FCD subtypes compared to controls. In mMCD/FCD and tubers, lesional cortex and white matter showed increased vascular density compared to perilesional tissues. Moreover, cortical vessel density increased with longer epilepsy duration and older age at surgery while in controls it decreased with age. Our findings suggest for that the increase in white matter vascular density might be pathology-specific rather than a consequence of ongoing epileptic activity. Increased cortical vessel density with age and with longer epilepsy duration in mMCD/FCD's and tubers, however, could be a consequence of seizures.
AB - Recent studies suggested a possible association between malformations of cortical development and microvascular density. In this study we aimed to further elucidate the relation between microvascular density and cortical developmental abnormalities in a cohort of 97 patients with epilepsy and histologically proven mild malformation of cortical development (mMCD), focal cortical dysplasia (FCD) or tuberous sclerosis complex (TSC). Surgical tissue samples were analyzed with quantitative measures of vessel density, T-cell response, microglial activation and myelin content. Subsequently, the results were compared to an age- and localization matched control group. We observed an increase in microvasculature in white matter of TSC cortical tubers, which is linked to inflammatory response. No increase was seen in mMCD or FCD subtypes compared to controls. In mMCD/FCD and tubers, lesional cortex and white matter showed increased vascular density compared to perilesional tissues. Moreover, cortical vessel density increased with longer epilepsy duration and older age at surgery while in controls it decreased with age. Our findings suggest for that the increase in white matter vascular density might be pathology-specific rather than a consequence of ongoing epileptic activity. Increased cortical vessel density with age and with longer epilepsy duration in mMCD/FCD's and tubers, however, could be a consequence of seizures.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85075576172&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31770571
U2 - https://doi.org/10.1016/j.ijdevneu.2019.11.003
DO - https://doi.org/10.1016/j.ijdevneu.2019.11.003
M3 - Article
C2 - 31770571
SN - 0736-5748
VL - 79
SP - 96
EP - 104
JO - International journal of developmental neuroscience
JF - International journal of developmental neuroscience
ER -