TY - JOUR
T1 - Characterization of the human spr2 promoter
T2 - Induction after UV irradiation or TPA treatment and regulation during differentiation of cultured primary keratinocytes
AU - Gibbs, Susan
AU - Lohman, Frans
AU - Teubel, Wilma
AU - Putte, Pieter van De
AU - Backendorf, Claude
PY - 1990/8/11
Y1 - 1990/8/11
N2 - We have isolated genomic clones from several members of the UV and TPA inducible human spr2 gene-family in order to analyse the regulation of these genes at a molecular level. From one of these members, the spr2-1 gene, we have identified and sequenced the regulatory region. By using CAT fusion plasmids and a liposome mediated transfection procedure we show that the isolated promoter region contains all the cis-elements necessary for induced expression after UV irradiation or phorbolester treatment of cultured human keratinocytes. Additionally the spr2-1 promoter is shown to be regulated aswell during the normal process of keratinocyte differentiation. This makes the spr2-1 promoter sequence an ideal tool to study the molecular mechanisms by which environmental agents such as UV radiation and chemical tumor promoters interfere with normal gene expression during cell proliferation and differentiation.
AB - We have isolated genomic clones from several members of the UV and TPA inducible human spr2 gene-family in order to analyse the regulation of these genes at a molecular level. From one of these members, the spr2-1 gene, we have identified and sequenced the regulatory region. By using CAT fusion plasmids and a liposome mediated transfection procedure we show that the isolated promoter region contains all the cis-elements necessary for induced expression after UV irradiation or phorbolester treatment of cultured human keratinocytes. Additionally the spr2-1 promoter is shown to be regulated aswell during the normal process of keratinocyte differentiation. This makes the spr2-1 promoter sequence an ideal tool to study the molecular mechanisms by which environmental agents such as UV radiation and chemical tumor promoters interfere with normal gene expression during cell proliferation and differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0025148421&partnerID=8YFLogxK
U2 - https://doi.org/10.1093/nar/18.15.4401
DO - https://doi.org/10.1093/nar/18.15.4401
M3 - Article
C2 - 2388825
SN - 0305-1048
VL - 18
SP - 4401
EP - 4407
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 15
ER -