TY - JOUR
T1 - Characterization of Yeast Peroxisomes
T2 - Enrichment of Peroxisomal Fractions and Analysis of β-Oxidation Activity
AU - van Roermund, Carlo
AU - Hettema, Ewald
N1 - Publisher Copyright: © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Subcellular fractionation approaches have allowed for the identification of various functionally distinct organelles including peroxisomes. The methods enable enrichment of organelles and combined with downstream assays allow for the identification of biochemical functions, composition, and structural characteristics of these compartments. In this chapter, we describe the methods for differential centrifugation and Nycodenz gradients in the yeast Saccharomyces cerevisiae and describe assays for fatty acid β-oxidation in intact cells and in peroxisomal fractions.
AB - Subcellular fractionation approaches have allowed for the identification of various functionally distinct organelles including peroxisomes. The methods enable enrichment of organelles and combined with downstream assays allow for the identification of biochemical functions, composition, and structural characteristics of these compartments. In this chapter, we describe the methods for differential centrifugation and Nycodenz gradients in the yeast Saccharomyces cerevisiae and describe assays for fatty acid β-oxidation in intact cells and in peroxisomal fractions.
KW - Nycodenz gradient
KW - Subcellular fractionation
KW - β-oxidation
UR - http://www.scopus.com/inward/record.url?scp=85151043199&partnerID=8YFLogxK
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85151043199&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/36952195
U2 - https://doi.org/10.1007/978-1-0716-3048-8_22
DO - https://doi.org/10.1007/978-1-0716-3048-8_22
M3 - Article
C2 - 36952195
SN - 1064-3745
VL - 2643
SP - 321
EP - 331
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -