TY - JOUR
T1 - Chromosomal instability and progressive loss of chromosomes in HeLa cells during adaptation to hyperoxic growth conditions
AU - Gille, J. J.P.
AU - Joenje, H.
PY - 1989/7
Y1 - 1989/7
N2 - By sequential selection for resistance to stepwise increase levels of atmospheric O2, a genetic variant of HeLa cells was obtained capable of stable proliferation under an atmosphere containing 80% O2 (HeLa-80). This cell strain has previously been characterized in terms of growth characteristics, morphology and antioxidant status (Joenje et al., 1985). In an attempt to find cytogenic clues possibly related to the O2-tolerant character, metaphases of HeLa-80 cells were analyzed and compared to the parental (HeLa-20) strain. Numerical analysis revealed a progressive decrease in the number of chromosomes percell during selection for O2 resistance, from a modal number of 112 in HeLa-20 cells to 84 in HeLa-80 cells. Cytogenetic endpoints for genetic damage revealed increased frequencies in HeLa-80 cells of both chromosomal aberrations (29.7 versus 6.9% aberrant cells) and sister-chromatid exchanges (0.46 ± 0.13 versus 0.31 ± 0.10 SCE/chromosome). G-banded metphases failed to reveal cytogenetic evidence of gene amplification (homogeneously staining regions, double minutes) in the karyotype of HeLa-80 cells.
AB - By sequential selection for resistance to stepwise increase levels of atmospheric O2, a genetic variant of HeLa cells was obtained capable of stable proliferation under an atmosphere containing 80% O2 (HeLa-80). This cell strain has previously been characterized in terms of growth characteristics, morphology and antioxidant status (Joenje et al., 1985). In an attempt to find cytogenic clues possibly related to the O2-tolerant character, metaphases of HeLa-80 cells were analyzed and compared to the parental (HeLa-20) strain. Numerical analysis revealed a progressive decrease in the number of chromosomes percell during selection for O2 resistance, from a modal number of 112 in HeLa-20 cells to 84 in HeLa-80 cells. Cytogenetic endpoints for genetic damage revealed increased frequencies in HeLa-80 cells of both chromosomal aberrations (29.7 versus 6.9% aberrant cells) and sister-chromatid exchanges (0.46 ± 0.13 versus 0.31 ± 0.10 SCE/chromosome). G-banded metphases failed to reveal cytogenetic evidence of gene amplification (homogeneously staining regions, double minutes) in the karyotype of HeLa-80 cells.
KW - Chromosomes aberrations
KW - HeLa cells
KW - Oxygen tolerance
KW - Sister-chromatid exchanges
UR - http://www.scopus.com/inward/record.url?scp=0024454931&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0921-8734(89)90004-0
DO - https://doi.org/10.1016/0921-8734(89)90004-0
M3 - Article
C2 - 2770770
SN - 0921-8734
VL - 219
SP - 225
EP - 230
JO - Mutation Research DNAging
JF - Mutation Research DNAging
IS - 4
ER -