Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy

A. van der Ende; L. J. van Doorn; S. Rooijakkers; M. Feller; G. N. Tytgat; J. Dankert

doi:https://doi.org/10.1128/JCM.39.7.2648-2651.2001

Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy

A. van der Ende, L. J. van Doorn, S. Rooijakkers, M. Feller, G. N. Tytgat, J. Dankert

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Research output: Contribution to journal › Article › Academic › peer-review

46 Citations (Scopus)

Abstract

Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA. The resistant and susceptible colonies of each of the heterogeneous H. pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes. In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary H. pylori population is used to test for clarithromycin susceptibility

Original language	English
Pages (from-to)	2648-2651
Journal	Journal of clinical microbiology
Volume	39
Issue number	7
DOIs	https://doi.org/10.1128/JCM.39.7.2648-2651.2001
Publication status	Published - 2001

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https://doi.org/10.1128/JCM.39.7.2648-2651.2001

Cite this

van der Ende, A., van Doorn, L. J., Rooijakkers, S., Feller, M., Tytgat, G. N., & Dankert, J. (2001). Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy. Journal of clinical microbiology, 39(7), 2648-2651. https://doi.org/10.1128/JCM.39.7.2648-2651.2001

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title = "Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy",

abstract = "Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA. The resistant and susceptible colonies of each of the heterogeneous H. pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes. In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary H. pylori population is used to test for clarithromycin susceptibility",

author = "{van der Ende}, A. and {van Doorn}, {L. J.} and S. Rooijakkers and M. Feller and Tytgat, {G. N.} and J. Dankert",

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doi = "https://doi.org/10.1128/JCM.39.7.2648-2651.2001",

language = "English",

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van der Ende, A, van Doorn, LJ, Rooijakkers, S, Feller, M, Tytgat, GN & Dankert, J 2001, 'Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy', Journal of clinical microbiology, vol. 39, no. 7, pp. 2648-2651. https://doi.org/10.1128/JCM.39.7.2648-2651.2001

Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy. / van der Ende, A.; van Doorn, L. J.; Rooijakkers, S. et al.
In: Journal of clinical microbiology, Vol. 39, No. 7, 2001, p. 2648-2651.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy

AU - van der Ende, A.

AU - van Doorn, L. J.

AU - Rooijakkers, S.

AU - Feller, M.

AU - Tytgat, G. N.

AU - Dankert, J.

PY - 2001

Y1 - 2001

N2 - Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA. The resistant and susceptible colonies of each of the heterogeneous H. pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes. In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary H. pylori population is used to test for clarithromycin susceptibility

AB - Of the Helicobacter pylori populations from 976 patients, six contained clarithromycin-resistant as well as -susceptible colonies. In each heterogeneous H. pylori population, resistant H. pylori colonies harbored identical 23S ribosomal DNA (rDNA) mutations associated with clarithromycin resistance, while the susceptible H. pylori colonies all had wild-type 23S rDNA. The resistant and susceptible colonies of each of the heterogeneous H. pylori populations had identical randomly amplified polymorphic DNA-PCR genotypes. In conclusion, evaluation of antimicrobial susceptibility can be misinterpreted if only a single colony from the primary H. pylori population is used to test for clarithromycin susceptibility

U2 - https://doi.org/10.1128/JCM.39.7.2648-2651.2001

DO - https://doi.org/10.1128/JCM.39.7.2648-2651.2001

M3 - Article

C2 - 11427586

SN - 0095-1137

VL - 39

SP - 2648

EP - 2651

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 7

ER -

van der Ende A, van Doorn LJ, Rooijakkers S, Feller M, Tytgat GN, Dankert J. Clarithromycin-susceptible and -resistant Helicobacter pylori isolates with identical randomly amplified polymorphic DNA-PCR genotypes cultured from single gastric biopsy specimens prior to antibiotic therapy. Journal of clinical microbiology. 2001;39(7):2648-2651. doi: https://doi.org/10.1128/JCM.39.7.2648-2651.2001