TY - JOUR
T1 - Comparative analysis of the natriuretic peptide precursor gene cluster in vertebrates reveals loss of ANF and retention of CNP-3 in chicken
AU - Houweling, Arjan C.
AU - Somi, Semir
AU - Massink, Maarten P. G.
AU - Groenen, Martien A.
AU - Moorman, Antoon F. M.
AU - Christoffels, Vincent M.
PY - 2005/7
Y1 - 2005/7
N2 - We identified and characterized the chicken natriuretic peptide precursor gene cluster and found its organization to be highly conserved compared with the mammalian Nppb-Nppa cluster. However, phylogenetic analysis indicated that the putative chicken natriuretic peptide precursor genes are the homologues of CNP-3 and Nppb, respectively. Comparative expression analysis revealed that, in human, mouse, and rat hearts, Nppb is a novel marker for the differentiating working myocardium. Its expression pattern is strikingly similar to that of Nppa before birth, and diverges only after birth. In contrast, whereas the chicken Nppb gene expression profile resembled that of mammalian Nppb, the CNP-3 gene showed very limited expression in the heart, not resembling the pattern of either Nppa or Nppb. These results show that, in chicken, the Nppa gene has been lost from the natriuretic peptide precursor gene cluster, whereas the CNP-3 gene has been retained
AB - We identified and characterized the chicken natriuretic peptide precursor gene cluster and found its organization to be highly conserved compared with the mammalian Nppb-Nppa cluster. However, phylogenetic analysis indicated that the putative chicken natriuretic peptide precursor genes are the homologues of CNP-3 and Nppb, respectively. Comparative expression analysis revealed that, in human, mouse, and rat hearts, Nppb is a novel marker for the differentiating working myocardium. Its expression pattern is strikingly similar to that of Nppa before birth, and diverges only after birth. In contrast, whereas the chicken Nppb gene expression profile resembled that of mammalian Nppb, the CNP-3 gene showed very limited expression in the heart, not resembling the pattern of either Nppa or Nppb. These results show that, in chicken, the Nppa gene has been lost from the natriuretic peptide precursor gene cluster, whereas the CNP-3 gene has been retained
KW - ANF
KW - BNF
KW - CNP-3
KW - Cardiac
KW - Chicken
KW - Gene expression
KW - Human
KW - Murine
KW - Phylogenetic analysis
UR - http://www.scopus.com/inward/record.url?scp=20744453492&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/dvdy.20423
DO - https://doi.org/10.1002/dvdy.20423
M3 - Article
C2 - 15895405
SN - 1058-8388
VL - 233
SP - 1076
EP - 1082
JO - Developmental dynamics
JF - Developmental dynamics
IS - 3
ER -