TY - JOUR
T1 - Comparative OCT imaging of human esophagus
T2 - How well can we localize the muscularis mucosae?
AU - Çilesiz, Inci
AU - Fockens, Paul
AU - Kerindongo, Raphaela
AU - Faber, Dirk
AU - Tytgat, Guido
AU - Ten Kate, Febo
AU - Van Leeuwen, Ton
PY - 2002
Y1 - 2002
N2 - Early diagnosis with esophageal cancer limited to the mucosa will allow for local endoscopic treatment and improve prognosis. We compared with histology OCT images of healthy human esophageal tissue from two systems operating at 800 and 1275 nm to investigate which wavelength was best suited for detailed OCT imaging of the esophageal wall, and to localize the muscularis mucosae. Within an hour of surgical resection, an esophageal specimen was cleaned of excess blood and soaked in formalin for a minimum of 48 hours. In order to precisely localize the different layers of the esophageal wall on an OCT image, well-defined structures within the esophageal wall were sought. Following OCT imaging the specimen was prepared for routine histology. We observed that our 1275 nm system with 12 μm resolution was superior in terms of penetration. As compared to histology, the 4 μm resolution of our 800 nm system made fine details more visible. Using either system, a minimally trained eye could recognize the muscularis mucosae as a hypo-reflective layer. Although different conditions may apply in vivo, our ex vivo study paves the path to precise interpretation of OCT images of the esophageal wall.
AB - Early diagnosis with esophageal cancer limited to the mucosa will allow for local endoscopic treatment and improve prognosis. We compared with histology OCT images of healthy human esophageal tissue from two systems operating at 800 and 1275 nm to investigate which wavelength was best suited for detailed OCT imaging of the esophageal wall, and to localize the muscularis mucosae. Within an hour of surgical resection, an esophageal specimen was cleaned of excess blood and soaked in formalin for a minimum of 48 hours. In order to precisely localize the different layers of the esophageal wall on an OCT image, well-defined structures within the esophageal wall were sought. Following OCT imaging the specimen was prepared for routine histology. We observed that our 1275 nm system with 12 μm resolution was superior in terms of penetration. As compared to histology, the 4 μm resolution of our 800 nm system made fine details more visible. Using either system, a minimally trained eye could recognize the muscularis mucosae as a hypo-reflective layer. Although different conditions may apply in vivo, our ex vivo study paves the path to precise interpretation of OCT images of the esophageal wall.
KW - Esophageal cancer
KW - Histology
KW - Muscularis mucosae
KW - OCT imaging
KW - Optical biopsy
UR - http://www.scopus.com/inward/record.url?scp=0036401571&partnerID=8YFLogxK
U2 - https://doi.org/10.1117/12.470482
DO - https://doi.org/10.1117/12.470482
M3 - Article
SN - 0277-786X
VL - 4619
SP - 187
EP - 193
JO - Proceedings of SPIE-The International Society for Optical Engineering
JF - Proceedings of SPIE-The International Society for Optical Engineering
ER -