Abstract
Aim: To explore the feasibility of screening for periodontitis by measuring biomarkers, namely total proteolytic activity (TPA), matrix metalloproteinase (MMP)-8, chitinase, lysozyme or their combination, in saliva, oral rinse and gingival crevicular fluid (GCF). Material and methods: Subjects were recruited among healthy/gingivitis individuals and untreated periodontitis patients in Academic Centre for Dentistry Amsterdam (ACTA). All participants donated samples of unstimulated whole saliva, oral rinse and GCF. The protein concentrations and MMP-8 levels were determined by ELISA. Enzymatic activities were measured using appropriate fluorogenic substrates. Results: In oral rinse samples, periodontitis patients (n = 19) exhibited significantly higher concentrations of MMP-8 and TPA than controls (n = 20). MMP-8 in combination with chitinase explained 88% of the variance and assigned a subject to control or periodontitis group, with best accuracy (87.2%) in oral rinse. Conclusions: The combination of MMP-8 and chitinase in the current oral rinse procedure has the potential to discriminate periodontitis from periodontal health/gingivitis.
Original language | English |
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Pages (from-to) | 1250-1259 |
Number of pages | 10 |
Journal | Journal of clinical periodontology |
Volume | 48 |
Issue number | 9 |
Early online date | 16 May 2021 |
DOIs | |
Publication status | Published - Sept 2021 |
Keywords
- MMP-8
- biomarkers
- chitinase
- gingival crevicular fluid
- modelling
- oral rinse
- periodontitis
- principal component analysis (PCA)
- protease
- saliva