Comparison of Schistosoma mansoni Soluble Cercarial Antigens and Soluble Egg Antigens for Serodiagnosing Schistosome Infections

Huw Smith; Mike Doenhoff; Cara Aitken; Wendi Bailey; Minjun Ji; Emily Dawson; Henk Gilis; Grant Spence; Claire Alexander; Tom van Gool

doi:https://doi.org/10.1371/journal.pntd.0001815

Comparison of Schistosoma mansoni Soluble Cercarial Antigens and Soluble Egg Antigens for Serodiagnosing Schistosome Infections

Huw Smith, Mike Doenhoff, Cara Aitken, Wendi Bailey, Minjun Ji, Emily Dawson, Henk Gilis, Grant Spence, Claire Alexander, Tom van Gool

Research output: Contribution to journal › Article › Academic › peer-review

44 Citations (Scopus)

Abstract

A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid - SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating laboratories to diagnose S. mansoni and S. haematobium infections. In the fourth laboratory the performance of SmCTF was compared with that of S. japonicum egg antigens (SjSEA) in ELISA for detection of anti-S. japonicum antibodies. In all 4 laboratories the results given by SmCTF in ELISA were very similar to those given by the antigen preparation routinely used in the respective laboratory to detect anti-schistosome antibodies in human infection sera. In so far as the ELISA results from SmCTF are thus so little different from those given by schistosome egg antigens and also cheaper to produce, the former is a potentially useful new diagnostic aid for schistosomiasis

Original language	English
Pages (from-to)	e1815
Journal	PLoS neglected tropical diseases
Volume	6
Issue number	9
DOIs	https://doi.org/10.1371/journal.pntd.0001815
Publication status	Published - 2012

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https://doi.org/10.1371/journal.pntd.0001815

Cite this

@article{d091e11956a84603b444cb9c94ded20a,

title = "Comparison of Schistosoma mansoni Soluble Cercarial Antigens and Soluble Egg Antigens for Serodiagnosing Schistosome Infections",

abstract = "A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid - SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating laboratories to diagnose S. mansoni and S. haematobium infections. In the fourth laboratory the performance of SmCTF was compared with that of S. japonicum egg antigens (SjSEA) in ELISA for detection of anti-S. japonicum antibodies. In all 4 laboratories the results given by SmCTF in ELISA were very similar to those given by the antigen preparation routinely used in the respective laboratory to detect anti-schistosome antibodies in human infection sera. In so far as the ELISA results from SmCTF are thus so little different from those given by schistosome egg antigens and also cheaper to produce, the former is a potentially useful new diagnostic aid for schistosomiasis",

author = "Huw Smith and Mike Doenhoff and Cara Aitken and Wendi Bailey and Minjun Ji and Emily Dawson and Henk Gilis and Grant Spence and Claire Alexander and {van Gool}, Tom",

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T1 - Comparison of Schistosoma mansoni Soluble Cercarial Antigens and Soluble Egg Antigens for Serodiagnosing Schistosome Infections

AU - Smith, Huw

AU - Doenhoff, Mike

AU - Aitken, Cara

AU - Bailey, Wendi

AU - Ji, Minjun

AU - Dawson, Emily

AU - Gilis, Henk

AU - Spence, Grant

AU - Alexander, Claire

AU - van Gool, Tom

PY - 2012

Y1 - 2012

N2 - A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid - SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating laboratories to diagnose S. mansoni and S. haematobium infections. In the fourth laboratory the performance of SmCTF was compared with that of S. japonicum egg antigens (SjSEA) in ELISA for detection of anti-S. japonicum antibodies. In all 4 laboratories the results given by SmCTF in ELISA were very similar to those given by the antigen preparation routinely used in the respective laboratory to detect anti-schistosome antibodies in human infection sera. In so far as the ELISA results from SmCTF are thus so little different from those given by schistosome egg antigens and also cheaper to produce, the former is a potentially useful new diagnostic aid for schistosomiasis

AB - A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid - SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating laboratories to diagnose S. mansoni and S. haematobium infections. In the fourth laboratory the performance of SmCTF was compared with that of S. japonicum egg antigens (SjSEA) in ELISA for detection of anti-S. japonicum antibodies. In all 4 laboratories the results given by SmCTF in ELISA were very similar to those given by the antigen preparation routinely used in the respective laboratory to detect anti-schistosome antibodies in human infection sera. In so far as the ELISA results from SmCTF are thus so little different from those given by schistosome egg antigens and also cheaper to produce, the former is a potentially useful new diagnostic aid for schistosomiasis

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DO - https://doi.org/10.1371/journal.pntd.0001815

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