Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A. T. Hesselink; A. J.C. Van Den Brule; Z. M.A. Groothuismink; M. Molano; J. Berkhof; C. J.L.M. Meijer; P. J.F. Snijders

doi:https://doi.org/10.1128/JCM.43.9.4868-4871.2005

Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A. T. Hesselink, A. J.C. Van Den Brule, Z. M.A. Groothuismink, M. Molano, J. Berkhof, C. J.L.M. Meijer, P. J.F. Snijders

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29 Citations (Scopus)

Abstract

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

Original language	English
Pages (from-to)	4868-4871
Number of pages	4
Journal	Journal of clinical microbiology
Volume	43
Issue number	9
DOIs	https://doi.org/10.1128/JCM.43.9.4868-4871.2005
Publication status	Published - 1 Sept 2005

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https://research.vumc.nl/ws/files/10478642/PMC1234115

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abstract = "We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.",

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AU - Van Den Brule, A. J.C.

AU - Groothuismink, Z. M.A.

AU - Molano, M.

AU - Berkhof, J.

AU - Meijer, C. J.L.M.

AU - Snijders, P. J.F.

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AB - We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

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Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

Abstract

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