TY - JOUR
T1 - Confounding factors from inducible systems for spatiotemporal gene expression regulation
AU - Wüst, Rob C. I.
AU - Houtkooper, Riekelt H.
AU - Auwerx, Johan
PY - 2020/5/19
Y1 - 2020/5/19
N2 - Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.
AB - Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.
UR - http://www.scopus.com/inward/record.url?scp=85084963245&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084963245&partnerID=8YFLogxK
U2 - https://doi.org/10.1083/jcb.202003031
DO - https://doi.org/10.1083/jcb.202003031
M3 - Review article
C2 - 32428200
SN - 0021-9525
VL - 219
SP - 1
EP - 4
JO - Journal of cell biology
JF - Journal of cell biology
IS - 7
M1 - e202003031
ER -
