Contrast-enhanced ultrasound for quantification of tissue perfusion in humans

Anna L. Emanuel, Rick I. Meijer, Erik van Poelgeest, Pien Spoor, Erik H. Serné, Etto C. Eringa

Research output: Contribution to journalReview articleAcademicpeer-review

39 Citations (Scopus)


Contrast-enhanced ultrasound is an imaging technique that can be used to quantify microvascular blood volume and blood flow of vital organs in humans. It relies on the use of microbubble contrast agents and ultrasound-based imaging of microbubbles. Over the past decades, both ultrasound contrast agents and experimental techniques to image them have rapidly improved, as did experience among investigators and clinicians. However, these improvements have not yet resulted in uniform guidelines for CEUS when it comes to quantification of tissue perfusion in humans, preventing its uniform and widespread use in research settings. The objective of this review is to provide a methodological overview of CEUS and its development, the influences of hardware and software settings, type and dosage of ultrasound contrast agent, and method of analysis on CEUS-derived perfusion data. Furthermore, we will discuss organ-specific imaging challenges, advantages, and limitations of CEUS.
Original languageEnglish
Article numbere12588
Issue number1
Early online date1 Jan 2019
Publication statusPublished - 1 Jan 2020


  • contrast-enhanced ultrasound
  • perfusion imaging

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