Coronaviruses in polarized epithelial cells

J. W. Rossen; C. P. Bekker; W. F. Voorhout; M. C. Horzinek; A. van der Ende; G. J. Strous; P. J. Rottier

Coronaviruses in polarized epithelial cells

J. W. Rossen, C. P. Bekker, W. F. Voorhout, M. C. Horzinek, A. van der Ende, G. J. Strous, P. J. Rottier

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Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively

Original language	English
Pages (from-to)	135-138
Journal	Advances in experimental medicine and biology
Volume	380
Publication status	Published - 1995

Cite this

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title = "Coronaviruses in polarized epithelial cells",

abstract = "Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively",

author = "Rossen, {J. W.} and Bekker, {C. P.} and Voorhout, {W. F.} and Horzinek, {M. C.} and {van der Ende}, A. and Strous, {G. J.} and Rottier, {P. J.}",

year = "1995",

language = "English",

volume = "380",

pages = "135--138",

journal = "Advances in experimental medicine and biology",

issn = "0065-2598",

publisher = "Springer New York",

}

TY - JOUR

T1 - Coronaviruses in polarized epithelial cells

AU - Rossen, J. W.

AU - Bekker, C. P.

AU - Voorhout, W. F.

AU - Horzinek, M. C.

AU - van der Ende, A.

AU - Strous, G. J.

AU - Rottier, P. J.

PY - 1995

Y1 - 1995

N2 - Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively

AB - Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively

M3 - Article

C2 - 8830469

SN - 0065-2598

VL - 380

SP - 135

EP - 138

JO - Advances in experimental medicine and biology

JF - Advances in experimental medicine and biology

ER -