TY - JOUR
T1 - Curcumin-based-fluorescent probes targeting ALDH1A3 as a promising tool for glioblastoma precision surgery and early diagnosis
AU - Gelardi, Edoardo L. M.
AU - Caprioglio, Diego
AU - Colombo, Giorgia
AU - del Grosso, Erika
AU - Mazzoletti, Daniele
AU - Mattoteia, Daiana
AU - Salamone, Stefano
AU - Ferraris, Davide M.
AU - Aronica, Eleonora
AU - Nato, Giulia
AU - Buffo, Annalisa
AU - Rizzi, Menico
AU - Magrassi, Lorenzo
AU - Minassi, Alberto
AU - Garavaglia, Silvia
N1 - Funding Information: S.G., A.M. and D.M.F. acknowledge the University of Piemonte Orientale (grant Ricerca Ateneo FAR_2016 and FAR_2019) and ROCHE per la Ricerca 2017, for the financial support. L.M. was supported by Fondazione I.R.C.C.S. Policlinico S. Matteo Ricerca Corrente (codice ric. 08016019). S.G. and A.M. acknowledge Professor Fabrizio Condorelli for the stimulating scientific discussion regarding this paper. We acknowledge the HIS Mouse Facility of the Academic Medical Center, Amsterdam and the Bloemenhove Clinic (Heemstede, The Netherlands) for providing fetal tissues. We would also thank Dott. Alberto Azzalin for his help with rMarkII flow cytometer. We acknowledge “HGCC” Uppsala University, Department of Immunology, Genetics and Pathology and its unit HGCC, (Rudbecklaboratoriet, 751 85 Uppsala, Sweden) for providing Patient-derived glioblastoma cells. Funding Information: S.G., A.M. and D.M.F. acknowledge the University of Piemonte Orientale (grant Ricerca Ateneo FAR_2016 and FAR_2019) and ROCHE per la Ricerca 2017, for the financial support. L.M. was supported by Fondazione I.R.C.C.S. Policlinico S. Matteo Ricerca Corrente (codice ric. 08016019). S.G. and A.M. acknowledge Professor Fabrizio Condorelli for the stimulating scientific discussion regarding this paper. We acknowledge the HIS Mouse Facility of the Academic Medical Center, Amsterdam and the Bloemenhove Clinic (Heemstede, The Netherlands) for providing fetal tissues. We would also thank Dott. Alberto Azzalin for his help with rMarkII flow cytometer. We acknowledge “HGCC” Uppsala University, Department of Immunology, Genetics and Pathology and its unit HGCC, (Rudbecklaboratoriet, 751 85 Uppsala, Sweden) for providing Patient-derived glioblastoma cells. Publisher Copyright: © 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Glioblastoma (GBM) is the most aggressive primary brain tumour for which both effective treatments and efficient tools for an early-stage diagnosis are lacking. Herein, we present curcumin-based fluorescent probes that are able to bind to aldehyde dehydrogenase 1A3 (ALDH1A3), an enzyme overexpressed in glioma stem cells (GSCs) and associated with stemness and invasiveness of GBM. Two compounds are selective versus ALDH1A3, without showing any appreciable interaction with other ALDH1A isoenzymes. Indeed, their fluorescent signal is detectable only in our positive controls in vitro and absent in cells that lack ALDH1A3. Remarkably, in vivo, our Probe selectively accumulate in glioblastoma cells, allowing the identification of the growing tumour mass. The significant specificity of our compounds is the necessary premise for their further development into glioblastoma cells detecting probes to be possibly used during neurosurgical operations.
AB - Glioblastoma (GBM) is the most aggressive primary brain tumour for which both effective treatments and efficient tools for an early-stage diagnosis are lacking. Herein, we present curcumin-based fluorescent probes that are able to bind to aldehyde dehydrogenase 1A3 (ALDH1A3), an enzyme overexpressed in glioma stem cells (GSCs) and associated with stemness and invasiveness of GBM. Two compounds are selective versus ALDH1A3, without showing any appreciable interaction with other ALDH1A isoenzymes. Indeed, their fluorescent signal is detectable only in our positive controls in vitro and absent in cells that lack ALDH1A3. Remarkably, in vivo, our Probe selectively accumulate in glioblastoma cells, allowing the identification of the growing tumour mass. The significant specificity of our compounds is the necessary premise for their further development into glioblastoma cells detecting probes to be possibly used during neurosurgical operations.
UR - http://www.scopus.com/inward/record.url?scp=85137094917&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/s42003-022-03834-7
DO - https://doi.org/10.1038/s42003-022-03834-7
M3 - Article
C2 - 36050388
SN - 2399-3642
VL - 5
SP - 895
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 895
ER -