Abstract
In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.
Original language | English |
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Pages (from-to) | 6360-4 |
Number of pages | 5 |
Journal | Journal of Immunology |
Volume | 163 |
Issue number | 12 |
Publication status | Published - 15 Dec 1999 |
Keywords
- Antigens, Surface/isolation & purification
- Cells, Cultured
- Cysteine/metabolism
- Disulfides/metabolism
- Epitopes/isolation & purification
- Female
- H-Y Antigen/isolation & purification
- HLA-A Antigens/immunology
- Humans
- Male
- Mass Spectrometry/methods
- Methionine/metabolism
- X Chromosome/genetics
- Y Chromosome/genetics