TY - JOUR
T1 - Cyclooxygenase-2 polymorphisms and the risk of esophageal adeno- or squamous cell carcinoma
AU - Kristinsson, Jón O.
AU - van Westerveld, Paul
AU - te Morsche, Rene H. M.
AU - Roelofs, Hennie M. J.
AU - Wobbes, T.
AU - Witteman, Ben J. M.
AU - Tan, Adriaan C. I. T. L.
AU - van Oijen, Martijn G. H.
AU - Jansen, Jan B. M. J.
AU - Peters, Wilbert H. M.
PY - 2009
Y1 - 2009
N2 - AIM: To determine whether -1195 A -> G and/or -765 G -> C polymorphisms in Cyclooxygenase-2 (COX-2) may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS: Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios (OR) and 95% confidence intervals (CI) were estimated. RESULTS: The distribution of the -1195A -> G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma (OR = 3.85, 95% Cl: 1.45-10.3) compared with the -1195 AA genotype as a reference. The -765 G -> C genotype distribution was not different between the two groups. The GG/GG haplotype was present more often in esophageal adenocarcinoma patients than in controls (OR = 3.45, 95% CI: 1.24-9.58; with AG/AG as a reference). The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION: Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma. (C) 2009 The WJG Press and Baishideng. All rights reserved
AB - AIM: To determine whether -1195 A -> G and/or -765 G -> C polymorphisms in Cyclooxygenase-2 (COX-2) may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS: Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios (OR) and 95% confidence intervals (CI) were estimated. RESULTS: The distribution of the -1195A -> G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma (OR = 3.85, 95% Cl: 1.45-10.3) compared with the -1195 AA genotype as a reference. The -765 G -> C genotype distribution was not different between the two groups. The GG/GG haplotype was present more often in esophageal adenocarcinoma patients than in controls (OR = 3.45, 95% CI: 1.24-9.58; with AG/AG as a reference). The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION: Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma. (C) 2009 The WJG Press and Baishideng. All rights reserved
U2 - https://doi.org/10.3748/wjg.15.3493
DO - https://doi.org/10.3748/wjg.15.3493
M3 - Article
C2 - 19630103
SN - 1007-9327
VL - 15
SP - 3493
EP - 3497
JO - World journal of gastroenterology
JF - World journal of gastroenterology
IS - 28
ER -