TY - JOUR
T1 - Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells
AU - Rodermond, Hans M.
AU - ten Cate, Rosemarie
AU - Haveman, Jaap
AU - van Kuilenburg, Andre
AU - Medema, Jan Paul
AU - van Bree, Chris
AU - Franken, Nicolaas A. P.
PY - 2010
Y1 - 2010
N2 - The search for agents that enhance the effect of ionizing radiation has been an object of study for decades In this study, the sensitizing properties of cyclopentenylcytosine (CPEC) on radiation and cisplatin-induced radiosensitization in human squamous lung carcinoma cells were investigated Human lung tumour SW-1573 cells (SWp, parental, SWg, gemcitabine-resistant) were incubated with CPEC and cisplatin and subsequently irradiated with different doses of gamma-rays Clonogenic survival was determined to measure the effectiveness of the treatments CPEC (1 or 2 mu M) treatment for 4 h decreased the plating efficiency to 75 and 50% in SWp and SWg cells, respectively In the SWg cells, 0 1 and 1 mu M CPEC for 4 h enhanced the cell killing effect of cisplatin However, an increase was not noted in the SWp cells Due to the moderate, toxicity of 1 mu M for 4 h, this CPEC dose was used in the radiosensitization experiments However, CPEC neither radiosensitized the lung tumour cells nor enhanced the radiosensitizing effect of cisplatin A 2-h incubation with 4 mu M cisplatin also decreased the plating efficiency to 75-80% in the two cell lines Using this cisplatin dose, radiosensitization was obtained in the two cell lines Although cisplatin treatment clearly radiosensitized the lung tumour cells CPEC treatment did not Cisplatin-induced radiosensitization was also not enhanced by CPEC
AB - The search for agents that enhance the effect of ionizing radiation has been an object of study for decades In this study, the sensitizing properties of cyclopentenylcytosine (CPEC) on radiation and cisplatin-induced radiosensitization in human squamous lung carcinoma cells were investigated Human lung tumour SW-1573 cells (SWp, parental, SWg, gemcitabine-resistant) were incubated with CPEC and cisplatin and subsequently irradiated with different doses of gamma-rays Clonogenic survival was determined to measure the effectiveness of the treatments CPEC (1 or 2 mu M) treatment for 4 h decreased the plating efficiency to 75 and 50% in SWp and SWg cells, respectively In the SWg cells, 0 1 and 1 mu M CPEC for 4 h enhanced the cell killing effect of cisplatin However, an increase was not noted in the SWp cells Due to the moderate, toxicity of 1 mu M for 4 h, this CPEC dose was used in the radiosensitization experiments However, CPEC neither radiosensitized the lung tumour cells nor enhanced the radiosensitizing effect of cisplatin A 2-h incubation with 4 mu M cisplatin also decreased the plating efficiency to 75-80% in the two cell lines Using this cisplatin dose, radiosensitization was obtained in the two cell lines Although cisplatin treatment clearly radiosensitized the lung tumour cells CPEC treatment did not Cisplatin-induced radiosensitization was also not enhanced by CPEC
U2 - https://doi.org/10.3892/ol_00000095
DO - https://doi.org/10.3892/ol_00000095
M3 - Article
C2 - 22966339
SN - 1792-1074
VL - 1
SP - 537
EP - 540
JO - Oncology Letters
JF - Oncology Letters
IS - 3
ER -