Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration

B. Daniel Lam, Eloise C. Anthony, Peter L. Hordijk

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25 Citations (Scopus)


The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(ΔNLS), however, we found that expression of SET and SET(ΔNLS) increases the levels of the MAP kinases ERK1 and ERK2
Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalFEBS letters
Issue number2
Publication statusPublished - 16 Jan 2013


  • Cell migration
  • Cell spreading
  • Rac1
  • SET/I2PP2A

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