Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration

B. Daniel Lam; Eloise C. Anthony; Peter L. Hordijk

doi:https://doi.org/10.1016/j.febslet.2012.11.013

Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration

B. Daniel Lam, Eloise C. Anthony, Peter L. Hordijk

Research output: Contribution to journal › Article › Academic › peer-review

25 Citations (Scopus)

Abstract

The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(ΔNLS), however, we found that expression of SET and SET(ΔNLS) increases the levels of the MAP kinases ERK1 and ERK2

Original language	English
Pages (from-to)	111-119
Number of pages	9
Journal	FEBS letters
Volume	587
Issue number	2
DOIs	https://doi.org/10.1016/j.febslet.2012.11.013
Publication status	Published - 16 Jan 2013

Keywords

Cell migration
Cell spreading
Rac1
SET/I2PP2A

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https://doi.org/10.1016/j.febslet.2012.11.013

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title = "Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration",

abstract = "The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(ΔNLS), however, we found that expression of SET and SET(ΔNLS) increases the levels of the MAP kinases ERK1 and ERK2",

keywords = "Cell migration, Cell spreading, Rac1, SET/I2PP2A",

author = "Lam, {B. Daniel} and Anthony, {Eloise C.} and Hordijk, {Peter L.}",

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T1 - Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration

AU - Lam, B. Daniel

AU - Anthony, Eloise C.

AU - Hordijk, Peter L.

PY - 2013/1/16

Y1 - 2013/1/16

N2 - The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(ΔNLS), however, we found that expression of SET and SET(ΔNLS) increases the levels of the MAP kinases ERK1 and ERK2

AB - The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied by an increase in the number and frequency of membrane ruffles. Pharmacological inhibition of PP2A did not mimic the effects of SET(ΔNLS), however, we found that expression of SET and SET(ΔNLS) increases the levels of the MAP kinases ERK1 and ERK2

KW - Cell migration

KW - Cell spreading

KW - Rac1

KW - SET/I2PP2A

UR - http://www.scopus.com/inward/record.url?scp=84872115395&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.febslet.2012.11.013

DO - https://doi.org/10.1016/j.febslet.2012.11.013

M3 - Article

C2 - 23195690

SN - 0014-5793

VL - 587

SP - 111

EP - 119

JO - FEBS letters

JF - FEBS letters

IS - 2

ER -

Cytoplasmic targeting of the proto-oncogene SET promotes cell spreading and migration

Abstract

Keywords

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