DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2

Juan J. García-Vallejo; Ellis van Liempt; Paula da Costa Martins; Cora Beckers; Bert van het Hof; Sonja I. Gringhuis; Jaap-Jan Zwaginga; Willem van Dijk; Teunis B. H. Geijtenbeek; Yvette van Kooyk; Irma van Die; P.A.G. Liempt; JJ Zwaginga

doi:https://doi.org/10.1016/j.molimm.2007.11.001

DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2

Juan J. García-Vallejo, Ellis van Liempt, Paula da Costa Martins, Cora Beckers, Bert van het Hof, Sonja I. Gringhuis, Jaap-Jan Zwaginga, Willem van Dijk, Teunis B. H. Geijtenbeek, Yvette van Kooyk, Irma van Die, P.A.G. Liempt, JJ Zwaginga

Research output: Contribution to journal › Article › Academic › peer-review

59 Citations (Scopus)

Abstract

Immature dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs mature and migrate to the secondary lymphoid organs where they initiate immune responses. DC-SIGN is a DC-specific C-type lectin that acts both as a pattern recognition receptor and as an adhesion molecule. As an adhesion molecule, DC-SIGN is able to mediate rolling and adhesion over endothelial cells under shear flow. In this study, we show that the binding partner of DC-SIGN on endothelial cells is the glycan epitope Lewis(Y) (Le(Y)), expressed on ICAM-2. The interaction between DC-SIGN on dendritic cells and ICAM-2 on endothelial cells is strictly glycan-specific. ICAM-2 expressed on CHO cells only served as a ligand for DC-SIGN when properly glycosylated, underscoring its function as a scaffolding protein. The expression of Le(Y) in endothelial cells is directed by the enzyme FUT1. Silencing of FUT1 results in a decrease in the rolling and adhesion of immature DCs over endothelial cells. The identification of Le(Y) as the carbohydrate ligand of DC-SIGN in endothelial cells opens new possibilities for the manipulation of DC migration

Original language	English
Pages (from-to)	2359-2369
Journal	Molecular immunology
Volume	45
Issue number	8
DOIs	https://doi.org/10.1016/j.molimm.2007.11.001
Publication status	Published - 2008

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Cite this

García-Vallejo, J. J., van Liempt, E., da Costa Martins, P., Beckers, C., van het Hof, B., Gringhuis, S. I., Zwaginga, J.-J., van Dijk, W., Geijtenbeek, T. B. H., van Kooyk, Y., van Die, I., Liempt, P. A. G., & Zwaginga, JJ. (2008). DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2. Molecular immunology, 45(8), 2359-2369. https://doi.org/10.1016/j.molimm.2007.11.001

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title = "DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2",

abstract = "Immature dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs mature and migrate to the secondary lymphoid organs where they initiate immune responses. DC-SIGN is a DC-specific C-type lectin that acts both as a pattern recognition receptor and as an adhesion molecule. As an adhesion molecule, DC-SIGN is able to mediate rolling and adhesion over endothelial cells under shear flow. In this study, we show that the binding partner of DC-SIGN on endothelial cells is the glycan epitope Lewis(Y) (Le(Y)), expressed on ICAM-2. The interaction between DC-SIGN on dendritic cells and ICAM-2 on endothelial cells is strictly glycan-specific. ICAM-2 expressed on CHO cells only served as a ligand for DC-SIGN when properly glycosylated, underscoring its function as a scaffolding protein. The expression of Le(Y) in endothelial cells is directed by the enzyme FUT1. Silencing of FUT1 results in a decrease in the rolling and adhesion of immature DCs over endothelial cells. The identification of Le(Y) as the carbohydrate ligand of DC-SIGN in endothelial cells opens new possibilities for the manipulation of DC migration",

author = "Garc{\'i}a-Vallejo, {Juan J.} and {van Liempt}, Ellis and {da Costa Martins}, Paula and Cora Beckers and {van het Hof}, Bert and Gringhuis, {Sonja I.} and Jaap-Jan Zwaginga and {van Dijk}, Willem and Geijtenbeek, {Teunis B. H.} and {van Kooyk}, Yvette and {van Die}, Irma and P.A.G. Liempt and JJ Zwaginga",

year = "2008",

doi = "https://doi.org/10.1016/j.molimm.2007.11.001",

language = "English",

volume = "45",

pages = "2359--2369",

journal = "Molecular immunology",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "8",

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García-Vallejo, JJ, van Liempt, E, da Costa Martins, P, Beckers, C, van het Hof, B, Gringhuis, SI, Zwaginga, J-J, van Dijk, W, Geijtenbeek, TBH , van Kooyk, Y, van Die, I, Liempt, PAG & Zwaginga, JJ 2008, 'DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2', Molecular immunology, vol. 45, no. 8, pp. 2359-2369. https://doi.org/10.1016/j.molimm.2007.11.001

DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2. / García-Vallejo, Juan J.; van Liempt, Ellis; da Costa Martins, Paula et al.
In: Molecular immunology, Vol. 45, No. 8, 2008, p. 2359-2369.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - DC-SIGN mediates adhesion and rolling of dendritic cells on primary human umbilical vein endothelial cells through LewisY antigen expressed on ICAM-2

AU - García-Vallejo, Juan J.

AU - van Liempt, Ellis

AU - da Costa Martins, Paula

AU - Beckers, Cora

AU - van het Hof, Bert

AU - Gringhuis, Sonja I.

AU - Zwaginga, Jaap-Jan

AU - van Dijk, Willem

AU - Geijtenbeek, Teunis B. H.

AU - van Kooyk, Yvette

AU - van Die, Irma

AU - Liempt, P.A.G.

AU - Zwaginga, JJ

PY - 2008

Y1 - 2008

N2 - Immature dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs mature and migrate to the secondary lymphoid organs where they initiate immune responses. DC-SIGN is a DC-specific C-type lectin that acts both as a pattern recognition receptor and as an adhesion molecule. As an adhesion molecule, DC-SIGN is able to mediate rolling and adhesion over endothelial cells under shear flow. In this study, we show that the binding partner of DC-SIGN on endothelial cells is the glycan epitope Lewis(Y) (Le(Y)), expressed on ICAM-2. The interaction between DC-SIGN on dendritic cells and ICAM-2 on endothelial cells is strictly glycan-specific. ICAM-2 expressed on CHO cells only served as a ligand for DC-SIGN when properly glycosylated, underscoring its function as a scaffolding protein. The expression of Le(Y) in endothelial cells is directed by the enzyme FUT1. Silencing of FUT1 results in a decrease in the rolling and adhesion of immature DCs over endothelial cells. The identification of Le(Y) as the carbohydrate ligand of DC-SIGN in endothelial cells opens new possibilities for the manipulation of DC migration

AB - Immature dendritic cells (DCs) are recruited from blood into tissues to patrol for foreign antigens. After antigen uptake and processing, DCs mature and migrate to the secondary lymphoid organs where they initiate immune responses. DC-SIGN is a DC-specific C-type lectin that acts both as a pattern recognition receptor and as an adhesion molecule. As an adhesion molecule, DC-SIGN is able to mediate rolling and adhesion over endothelial cells under shear flow. In this study, we show that the binding partner of DC-SIGN on endothelial cells is the glycan epitope Lewis(Y) (Le(Y)), expressed on ICAM-2. The interaction between DC-SIGN on dendritic cells and ICAM-2 on endothelial cells is strictly glycan-specific. ICAM-2 expressed on CHO cells only served as a ligand for DC-SIGN when properly glycosylated, underscoring its function as a scaffolding protein. The expression of Le(Y) in endothelial cells is directed by the enzyme FUT1. Silencing of FUT1 results in a decrease in the rolling and adhesion of immature DCs over endothelial cells. The identification of Le(Y) as the carbohydrate ligand of DC-SIGN in endothelial cells opens new possibilities for the manipulation of DC migration

U2 - https://doi.org/10.1016/j.molimm.2007.11.001

DO - https://doi.org/10.1016/j.molimm.2007.11.001

M3 - Article

C2 - 18155766

SN - 0161-5890

VL - 45

SP - 2359

EP - 2369

JO - Molecular immunology

JF - Molecular immunology

IS - 8

ER -