DCs facilitate B cell responses against microbial DNA via DC-SIGN

Joris K. Sprokholt; Marieke H. Heineke; Tanja M. Kaptein; John L. van Hamme; Teunis B. H. Geijtenbeek

doi:https://doi.org/10.1371/journal.pone.0185580

DCs facilitate B cell responses against microbial DNA via DC-SIGN

Joris K. Sprokholt, Marieke H. Heineke, Tanja M. Kaptein, John L. van Hamme, Teunis B. H. Geijtenbeek

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Microbial DNA is highly immunostimulatory and is sensed by endosomal pattern recognition receptors after release from internalized microbes. It is unclear how extracellular DNA released from dead microbes is delivered to endosomal PRRs to induce immune responses. Here we have investigated the ability of DCs to bind and internalize extracellular E.coli DNA as well as synthetic DNA. DCs internalized E.coli and synthetic DNA, which was dependent on the C-type lectin receptor DC-SIGN. Notably, endosomal uptake of DNA by DCs enhanced TLR9-dependent responses of B cells against DNA. Hence, we have identified DC-SIGN as a cell surface receptor for DNA that facilitates immune responses directed against DNA

Original language	English
Pages (from-to)	e0185580
Journal	PLOS ONE
Volume	12
Issue number	10
DOIs	https://doi.org/10.1371/journal.pone.0185580
Publication status	Published - 2017

Keywords

B-Lymphocytes/immunology
Cell Adhesion Molecules/physiology
Cytokines/biosynthesis
DNA, Bacterial/immunology
Dendritic Cells/immunology
Escherichia coli/genetics
Humans
Interferon Type I/biosynthesis
Lectins, C-Type/physiology
Receptors, Cell Surface/physiology

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Cite this

@article{dd44a6aaef414888bbe2e4da9831903e,

title = "DCs facilitate B cell responses against microbial DNA via DC-SIGN",

abstract = "Microbial DNA is highly immunostimulatory and is sensed by endosomal pattern recognition receptors after release from internalized microbes. It is unclear how extracellular DNA released from dead microbes is delivered to endosomal PRRs to induce immune responses. Here we have investigated the ability of DCs to bind and internalize extracellular E.coli DNA as well as synthetic DNA. DCs internalized E.coli and synthetic DNA, which was dependent on the C-type lectin receptor DC-SIGN. Notably, endosomal uptake of DNA by DCs enhanced TLR9-dependent responses of B cells against DNA. Hence, we have identified DC-SIGN as a cell surface receptor for DNA that facilitates immune responses directed against DNA",

keywords = "B-Lymphocytes/immunology, Cell Adhesion Molecules/physiology, Cytokines/biosynthesis, DNA, Bacterial/immunology, Dendritic Cells/immunology, Escherichia coli/genetics, Humans, Interferon Type I/biosynthesis, Lectins, C-Type/physiology, Receptors, Cell Surface/physiology",

author = "Sprokholt, {Joris K.} and Heineke, {Marieke H.} and Kaptein, {Tanja M.} and {van Hamme}, {John L.} and Geijtenbeek, {Teunis B. H.}",

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T1 - DCs facilitate B cell responses against microbial DNA via DC-SIGN

AU - Sprokholt, Joris K.

AU - Heineke, Marieke H.

AU - Kaptein, Tanja M.

AU - van Hamme, John L.

AU - Geijtenbeek, Teunis B. H.

PY - 2017

Y1 - 2017

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AB - Microbial DNA is highly immunostimulatory and is sensed by endosomal pattern recognition receptors after release from internalized microbes. It is unclear how extracellular DNA released from dead microbes is delivered to endosomal PRRs to induce immune responses. Here we have investigated the ability of DCs to bind and internalize extracellular E.coli DNA as well as synthetic DNA. DCs internalized E.coli and synthetic DNA, which was dependent on the C-type lectin receptor DC-SIGN. Notably, endosomal uptake of DNA by DCs enhanced TLR9-dependent responses of B cells against DNA. Hence, we have identified DC-SIGN as a cell surface receptor for DNA that facilitates immune responses directed against DNA

KW - B-Lymphocytes/immunology

KW - Cell Adhesion Molecules/physiology

KW - Cytokines/biosynthesis

KW - DNA, Bacterial/immunology

KW - Dendritic Cells/immunology

KW - Escherichia coli/genetics

KW - Humans

KW - Interferon Type I/biosynthesis

KW - Lectins, C-Type/physiology

KW - Receptors, Cell Surface/physiology

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DO - https://doi.org/10.1371/journal.pone.0185580

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