Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels

Rabeil Sakina, Aida Llucià-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota, Lorenzo Moroni

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

5 Citations (Scopus)


The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.
Original languageEnglish
Title of host publicationMethods in Cell Biology
EditorsDavid Caballero, Subhas C. Kundu, Rui L. Reis
PublisherAcademic Press Inc.
Number of pages19
ISBN (Print)9780128201749
Publication statusPublished - 2020

Publication series

NameMethods in Cell Biology


  • Cardiac
  • Decellularized
  • Extracellular matrix
  • Hydrogel
  • Tissue engineering

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