TY - JOUR
T1 - Demonstration of interleukin-1β in Lewis rat brain during experimental allergic encephalomyelitis by immunocytochemistry at the light and ultrastructural level
AU - Bauer, Jan
AU - Berkenbosch, Frank
AU - Van Dam, Anne Marie
AU - Dijkstra, Christine D.
PY - 1993/10
Y1 - 1993/10
N2 - Interleukin-1β (IL-1) is a cytokine which exerts many biological effects during inflammation. In the present study, experimental allergic encephalomyelitis (EAE) was induced in Lewis rats. During the various stages of EAE, the presence of IL-1 in the brain was investigated using immunocytochemistry at both the light and ultrastructural level. Ten days after immunization, IL-1 immunoreactivity was found in brains of animals which at this time showed mild clinical signs. Outside the blood-brain barrier, IL-1 was localized in the cytoplasm of meningeal macrophages and perivascular cells. Within the brain parenchyma, IL-1 immunoreactivity was distributed in perivascular lesions in the cytoplasm of infiltrated macrophages and activated microglia. On day 13, animals had developed a full blown EAE. At this stage the number of lesions with IL-1-positive cells had increased. In the remission phase (day 25), lesions with IL-1-positive cells could still be detected but were less pronounced as compared to day 13. Other presumptive IL-1-producing cell types like endothelial cells or astrocytes were, at non of the various stages, found to stain for IL-1.
AB - Interleukin-1β (IL-1) is a cytokine which exerts many biological effects during inflammation. In the present study, experimental allergic encephalomyelitis (EAE) was induced in Lewis rats. During the various stages of EAE, the presence of IL-1 in the brain was investigated using immunocytochemistry at both the light and ultrastructural level. Ten days after immunization, IL-1 immunoreactivity was found in brains of animals which at this time showed mild clinical signs. Outside the blood-brain barrier, IL-1 was localized in the cytoplasm of meningeal macrophages and perivascular cells. Within the brain parenchyma, IL-1 immunoreactivity was distributed in perivascular lesions in the cytoplasm of infiltrated macrophages and activated microglia. On day 13, animals had developed a full blown EAE. At this stage the number of lesions with IL-1-positive cells had increased. In the remission phase (day 25), lesions with IL-1-positive cells could still be detected but were less pronounced as compared to day 13. Other presumptive IL-1-producing cell types like endothelial cells or astrocytes were, at non of the various stages, found to stain for IL-1.
KW - Astrocytes
KW - Experimental allergic encephalomyelitis
KW - Interleukin-1
KW - Macrophages
KW - Microglia
UR - http://www.scopus.com/inward/record.url?scp=0027331778&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/0165-5728(93)90053-2
DO - https://doi.org/10.1016/0165-5728(93)90053-2
M3 - Article
C2 - 8227304
SN - 0165-5728
VL - 48
SP - 13
EP - 21
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1
ER -