Dendritic cells as accessory cells in antigen-specific murine T lymphocyte proliferation in vitro

M. B. Teunissen; M. L. Kapsenberg; F. E. Stiekema; H. G. Keizer

doi:https://doi.org/10.1016/0022-1759(85)90190-5

Dendritic cells as accessory cells in antigen-specific murine T lymphocyte proliferation in vitro

M. B. Teunissen, M. L. Kapsenberg, F. E. Stiekema, H. G. Keizer

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Abstract

The role of dendritic cells in antigen-induced murine T lymphocyte activation was studied by addition of purified dendritic cells to purified lymph node T lymphocytes from ovalbumin-primed mice. In the presence of the priming antigen T cells generated an antigen-specific response. The response was at least 3-fold higher with the use of a modified IMDM culture medium. The complete requirement for accessory cells was demonstrated only when nylon wool-purified T lymphocytes were thoroughly depleted of Ia antigen-expressing cells. Dendritic cells as well as peritoneal exudate macrophages were equally effective as antigen-presenting cells

Original language	English
Pages (from-to)	131-138
Journal	Journal of immunological methods
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(85)90190-5
Publication status	Published - 1985

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https://doi.org/10.1016/0022-1759(85)90190-5

Cite this

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title = "Dendritic cells as accessory cells in antigen-specific murine T lymphocyte proliferation in vitro",

abstract = "The role of dendritic cells in antigen-induced murine T lymphocyte activation was studied by addition of purified dendritic cells to purified lymph node T lymphocytes from ovalbumin-primed mice. In the presence of the priming antigen T cells generated an antigen-specific response. The response was at least 3-fold higher with the use of a modified IMDM culture medium. The complete requirement for accessory cells was demonstrated only when nylon wool-purified T lymphocytes were thoroughly depleted of Ia antigen-expressing cells. Dendritic cells as well as peritoneal exudate macrophages were equally effective as antigen-presenting cells",

author = "Teunissen, {M. B.} and Kapsenberg, {M. L.} and Stiekema, {F. E.} and Keizer, {H. G.}",

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AU - Kapsenberg, M. L.

AU - Stiekema, F. E.

AU - Keizer, H. G.

PY - 1985

Y1 - 1985

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AB - The role of dendritic cells in antigen-induced murine T lymphocyte activation was studied by addition of purified dendritic cells to purified lymph node T lymphocytes from ovalbumin-primed mice. In the presence of the priming antigen T cells generated an antigen-specific response. The response was at least 3-fold higher with the use of a modified IMDM culture medium. The complete requirement for accessory cells was demonstrated only when nylon wool-purified T lymphocytes were thoroughly depleted of Ia antigen-expressing cells. Dendritic cells as well as peritoneal exudate macrophages were equally effective as antigen-presenting cells

U2 - https://doi.org/10.1016/0022-1759(85)90190-5

DO - https://doi.org/10.1016/0022-1759(85)90190-5

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