Detection of HIV-1 DNA in leukocytes using a commercially available assay

H. L. Zaaijer; H. T. Cuypers; H. W. Reesink; J. H. ten Veen; S. C. Schoon-Visser; P. N. Lelie

doi:https://doi.org/10.1111/j.1423-0410.1994.tb00282.x

Detection of HIV-1 DNA in leukocytes using a commercially available assay

H. L. Zaaijer, H. T. Cuypers, H. W. Reesink, J. H. ten Veen, S. C. Schoon-Visser, P. N. Lelie

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Research output: Contribution to journal › Comment/Letter to the editor › Academic

6 Citations (Scopus)

Abstract

Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection

Original language	English
Pages (from-to)	78-80
Journal	Vox sanguinis
Volume	66
Issue number	1
DOIs	https://doi.org/10.1111/j.1423-0410.1994.tb00282.x
Publication status	Published - 1994

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https://doi.org/10.1111/j.1423-0410.1994.tb00282.x

Cite this

@article{79e5c47e08914e3a997e7f4eb898151d,

title = "Detection of HIV-1 DNA in leukocytes using a commercially available assay",

abstract = "Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection",

author = "Zaaijer, {H. L.} and Cuypers, {H. T.} and Reesink, {H. W.} and {ten Veen}, {J. H.} and Schoon-Visser, {S. C.} and Lelie, {P. N.}",

year = "1994",

doi = "https://doi.org/10.1111/j.1423-0410.1994.tb00282.x",

language = "English",

volume = "66",

pages = "78--80",

journal = "Vox sanguinis",

issn = "0042-9007",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Detection of HIV-1 DNA in leukocytes using a commercially available assay

AU - Zaaijer, H. L.

AU - Cuypers, H. T.

AU - Reesink, H. W.

AU - ten Veen, J. H.

AU - Schoon-Visser, S. C.

AU - Lelie, P. N.

PY - 1994

Y1 - 1994

N2 - Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection

AB - Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infection

U2 - https://doi.org/10.1111/j.1423-0410.1994.tb00282.x

DO - https://doi.org/10.1111/j.1423-0410.1994.tb00282.x

M3 - Comment/Letter to the editor

C2 - 7908474

SN - 0042-9007

VL - 66

SP - 78

EP - 80

JO - Vox sanguinis

JF - Vox sanguinis

IS - 1

ER -