TY - JOUR
T1 - Determinants of DNA yield and purity collected with buccal cell samples
AU - van Wieren-de Wijer, D. B. M. A.
AU - Maitland-van der Zee, A. H.
AU - de Boer, A. [=Anthonius]
AU - Belitser, S. V.
AU - Kroon, A. A.
AU - de Leeuw, P. W.
AU - Schiffers, P.
AU - Janssen, R. G. J. H.
AU - van Duijn, C. M.
AU - Stricker, B. H. C. H.
AU - Klungel, O. H.
PY - 2009
Y1 - 2009
N2 - Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 mu g (median 54.3 mu g; mean 82.2 mu g +/- A SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 mu g) than those from women (median 44.2 mu g). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics
AB - Buccal cells are an important source of DNA in epidemiological studies, but little is known about factors that influence amount and purity of DNA. We assessed these factors in a self-administered buccal cell collection procedure, obtained with three cotton swabs. In 2,451 patients DNA yield and in 1,033 patients DNA purity was assessed. Total DNA yield ranged from 0.08 to 1078.0 mu g (median 54.3 mu g; mean 82.2 mu g +/- A SD 92.6). The median UV 260:280 ratio, was 1.95. Samples from men yielded significantly more DNA (median 58.7 mu g) than those from women (median 44.2 mu g). Diuretic drug users had significantly lower purity (median 1.92) compared to other antihypertensive drug users (1.95). One technician obtained significantly lower DNA yields. Older age was associated with lower DNA purity. In conclusion, DNA yield from buccal swabs was higher in men and DNA purity was associated with age and the use of diuretics
U2 - https://doi.org/10.1007/s10654-009-9388-x
DO - https://doi.org/10.1007/s10654-009-9388-x
M3 - Article
C2 - 19760105
SN - 0393-2990
VL - 24
SP - 677
EP - 682
JO - European Journal of Epidemiology
JF - European Journal of Epidemiology
IS - 11
ER -