Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

C. A. Knibbe; V. S. Koster; V. H. Deneer; R. M. Stuurman; P. F. Kuks; R. Lange

doi:https://doi.org/10.1016/S0378-4347(97)00571-9

Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

C. A. Knibbe, V. S. Koster, V. H. Deneer, R. M. Stuurman, P. F. Kuks, R. Lange

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Abstract

In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay

Original language	English
Pages (from-to)	305-310
Journal	Journal of chromatography. B, Biomedical sciences and applications
Volume	706
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(97)00571-9
Publication status	Published - 1998

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https://doi.org/10.1016/S0378-4347(97)00571-9

Cite this

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title = "Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection",

abstract = "In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay",

author = "Knibbe, {C. A.} and Koster, {V. S.} and Deneer, {V. H.} and Stuurman, {R. M.} and Kuks, {P. F.} and R. Lange",

year = "1998",

doi = "https://doi.org/10.1016/S0378-4347(97)00571-9",

language = "English",

volume = "706",

pages = "305--310",

journal = "Journal of chromatography. B, Biomedical sciences and applications",

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number = "2",

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T1 - Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

AU - Knibbe, C. A.

AU - Koster, V. S.

AU - Deneer, V. H.

AU - Stuurman, R. M.

AU - Kuks, P. F.

AU - Lange, R.

PY - 1998

Y1 - 1998

N2 - In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay

AB - In order to determine propofol in rat whole-blood samples of 50 microl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay

U2 - https://doi.org/10.1016/S0378-4347(97)00571-9

DO - https://doi.org/10.1016/S0378-4347(97)00571-9

M3 - Article

C2 - 9551817

SN - 1387-2273

VL - 706

SP - 305

EP - 310

JO - Journal of chromatography. B, Biomedical sciences and applications

JF - Journal of chromatography. B, Biomedical sciences and applications

IS - 2

ER -