TY - JOUR
T1 - Deuterium isotope effect on enantioselectivity in the Comamonas testosteroni quinohemoprotein alcohol dehydrogenase-catalyzed kinetic resolution of rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane, solketal
AU - Jongejan, Aldo
AU - Jongejan, Jaap A.
AU - Hagen, Wilfred R.
PY - 2003/4/11
Y1 - 2003/4/11
N2 - Isotopic substitution provides an effective tool to probe the mechanism of enzyme-catalyzed reactions. To our knowledge, kinetic isotope effects on the enantioselectivity of enzymes have not been reported. We investigated the effect of deuterium substitution on the enantiomeric ratio, E, of PQQ-containing quinohemoprotein alcohol dehydrogenase, QH-ADH, from Comamonas testosteroni in the ferricyanide-coupled kinetic resolution of rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane, solketal. Under otherwise identical conditions, we measured E=30 for solketal and E=6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4- 2H]dioxolane, d5-solketal. It is proposed that isotopic substitution affects the relative kinetic weights of the initial hydron/ deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex. The latter step becomes more important in the deuterated complex to the extent that the enantiomer discrimination in the first step is partially overruled.
AB - Isotopic substitution provides an effective tool to probe the mechanism of enzyme-catalyzed reactions. To our knowledge, kinetic isotope effects on the enantioselectivity of enzymes have not been reported. We investigated the effect of deuterium substitution on the enantiomeric ratio, E, of PQQ-containing quinohemoprotein alcohol dehydrogenase, QH-ADH, from Comamonas testosteroni in the ferricyanide-coupled kinetic resolution of rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane, solketal. Under otherwise identical conditions, we measured E=30 for solketal and E=6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4- 2H]dioxolane, d5-solketal. It is proposed that isotopic substitution affects the relative kinetic weights of the initial hydron/ deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex. The latter step becomes more important in the deuterated complex to the extent that the enantiomer discrimination in the first step is partially overruled.
KW - Alcohol dehydrogenase
KW - Comamonas testosteroni
KW - Deuterium kinetic isotope effect
KW - Enantioselectivity
KW - Kinetic resolution
KW - Pyrroloquinoline quinone
KW - Quinohemoprotein
KW - Solketal
UR - http://www.scopus.com/inward/record.url?scp=0037636437&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/S1570-9639(03)00073-6
DO - https://doi.org/10.1016/S1570-9639(03)00073-6
M3 - Article
C2 - 12686148
SN - 1570-9639
VL - 1647
SP - 297
EP - 302
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 1-2
ER -