Abstract
For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.
Original language | English |
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Pages (from-to) | 493-504 |
Number of pages | 12 |
Journal | Immunity |
Volume | 7 |
Issue number | 4 |
Publication status | Published - Oct 1997 |
Keywords
- Animals
- Animals, Newborn
- Antigen-Presenting Cells/cytology
- B-Lymphocytes/cytology
- CD3 Complex/metabolism
- CD4-Positive T-Lymphocytes/cytology
- Cell Adhesion Molecules
- Cytotoxicity, Immunologic
- Fluorescent Antibody Technique, Indirect
- GTP-Binding Proteins/genetics
- Gene Expression
- Histocompatibility Antigens Class II/metabolism
- Immunity, Cellular
- Immunoglobulins/metabolism
- Integrins/metabolism
- Interleukin-2/pharmacology
- Killer Cells, Natural/cytology
- Leukocyte Common Antigens/analysis
- Leukopoiesis
- Lymph Nodes/cytology
- Lymphocyte Subsets/cytology
- Lymphotoxin-alpha/metabolism
- Lymphotoxin-beta
- Membrane Proteins/metabolism
- Mice
- Mice, Inbred AKR
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mucoproteins/metabolism
- RNA, Messenger/genetics
- Receptors, CXCR5
- Receptors, Chemokine
- Receptors, Cytokine/genetics
- Spleen/embryology
- T-Lymphocytes/cytology