Developing lymph nodes collect CD4+CD3- LTbeta+ cells that can differentiate to APC, NK cells, and follicular cells but not T or B cells

R E Mebius, P Rennert, I L Weissman

Research output: Contribution to journalArticleAcademicpeer-review

604 Citations (Scopus)

Abstract

For a brief period during fetal lymph node organogenesis in mice, lymph node postcapillary high endothelial venules surprisingly express the Peyer's patch addressin MAdCAM-1. This expression allows initial seeding of this incipient structure by two unusual lymphocyte populations selectively expressing the Peyer's patch homing receptor integrin alpha4beta7: CD4+CD3- oligolineage progenitors and TCR gammadelta+ T cells. We show here that CD4+CD3- cells are lineage-restricted progenitors that express surface lymphotoxin-beta (LTbeta) and the chemokine receptor BLR1 and that can become natural killer cells, dendritic antigen-presenting cells, and follicular cells of unknown outcome, but these cells do not become T or B lymphocytes. Since the necessity of lymphotoxin in lymphoid organ development has been shown, we propose that the novel subset of CD4+CD3-LTbeta+ fetal cells is instrumental in the development of lymphoid tissue architecture.

Original languageEnglish
Pages (from-to)493-504
Number of pages12
JournalImmunity
Volume7
Issue number4
Publication statusPublished - Oct 1997

Keywords

  • Animals
  • Animals, Newborn
  • Antigen-Presenting Cells/cytology
  • B-Lymphocytes/cytology
  • CD3 Complex/metabolism
  • CD4-Positive T-Lymphocytes/cytology
  • Cell Adhesion Molecules
  • Cytotoxicity, Immunologic
  • Fluorescent Antibody Technique, Indirect
  • GTP-Binding Proteins/genetics
  • Gene Expression
  • Histocompatibility Antigens Class II/metabolism
  • Immunity, Cellular
  • Immunoglobulins/metabolism
  • Integrins/metabolism
  • Interleukin-2/pharmacology
  • Killer Cells, Natural/cytology
  • Leukocyte Common Antigens/analysis
  • Leukopoiesis
  • Lymph Nodes/cytology
  • Lymphocyte Subsets/cytology
  • Lymphotoxin-alpha/metabolism
  • Lymphotoxin-beta
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mucoproteins/metabolism
  • RNA, Messenger/genetics
  • Receptors, CXCR5
  • Receptors, Chemokine
  • Receptors, Cytokine/genetics
  • Spleen/embryology
  • T-Lymphocytes/cytology

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