TY - JOUR
T1 - Development and fabrication of co-axially electrospun biomimetic periosteum with a decellularized periosteal ECM shell/PCL core structure to promote the repair of critical-sized bone defects
AU - Li, Shuyi
AU - Deng, Rongli
AU - Zou, Xuenong
AU - Rong, Qiong
AU - Shou, Jiali
AU - Rao, Zilong
AU - Wu, Wanqiu
AU - Wu, Gang
AU - Quan, Daping
AU - Zhou, Miao
AU - Forouzanfar, Tim
N1 - Funding Information: We acknowledged Dr. Yubo Tang for instructing us to harvest MSCs. This work was financially supported by the National Natural Science Foundation of China (Grant number 81671029 , 82001007 ), the National Major Science and Technology Project of China (Grant number 2016YFC1102900 ), the Guangzhou Science, Technology and Innovation Commission (Grant numbers 201803040008 ), and China Scholarship Council (No. 201908440308 ). Publisher Copyright: © 2022
PY - 2022/4/1
Y1 - 2022/4/1
N2 - Periosteum is crucial to the initial healing of bone defects because it provides a stable periosteum-specific microenvironment and abundant osteogenic cells towards bone repair. However, the usage of autologous periosteum is restricted for limited availability. Xenogeneic decellularized periosteum (DP) preserves periosteum-specific cues but the hardly-controlled physicochemical properties render it inconvenient to use widely. Our study aimed to fabricate a tissue-engineered periosteum (TEP) with a continuous periosteum-specifically bioactive surface and tailored physicochemical properties by co-axial electrospinning of poly(ε-caprolactone) (PCL)/periosteal decellularized extracellular matrix (dECM) to promote bone defects healing. Before use, DP was confirmed for effective removal of residual DNA and well preservation of biological components, e.g., collagen and glycosaminoglycans. After optimization of fabrication processes, PCL/dECM co-axially electrospun membrane (PEC) bore an exquisite core-shell structure, which was effectively equipped with composite advantages of dECM and PCL. Notably, PEC stood out in terms of tensile strength and long-term durability within a physiological environment compared to that of the dECM uniaxial electrospun membrane (ECM). Besides, PEC exhibited remarkably better cell proliferation, migration, bio-mineralization, and osteogenic properties as compared to that of the electrospun PCL membrane. Moreover, PEC-TEP could significantly enhance the recovery of critical-sized bone defects in rats than that of PCL-TEP. To the best of our knowledge, biomimetic PEC-TEP was fabricated and used for the first time to repair bone defects. This novel strategy is promising to fabricate a dECM-based advanced membrane for tissue engineering.
AB - Periosteum is crucial to the initial healing of bone defects because it provides a stable periosteum-specific microenvironment and abundant osteogenic cells towards bone repair. However, the usage of autologous periosteum is restricted for limited availability. Xenogeneic decellularized periosteum (DP) preserves periosteum-specific cues but the hardly-controlled physicochemical properties render it inconvenient to use widely. Our study aimed to fabricate a tissue-engineered periosteum (TEP) with a continuous periosteum-specifically bioactive surface and tailored physicochemical properties by co-axial electrospinning of poly(ε-caprolactone) (PCL)/periosteal decellularized extracellular matrix (dECM) to promote bone defects healing. Before use, DP was confirmed for effective removal of residual DNA and well preservation of biological components, e.g., collagen and glycosaminoglycans. After optimization of fabrication processes, PCL/dECM co-axially electrospun membrane (PEC) bore an exquisite core-shell structure, which was effectively equipped with composite advantages of dECM and PCL. Notably, PEC stood out in terms of tensile strength and long-term durability within a physiological environment compared to that of the dECM uniaxial electrospun membrane (ECM). Besides, PEC exhibited remarkably better cell proliferation, migration, bio-mineralization, and osteogenic properties as compared to that of the electrospun PCL membrane. Moreover, PEC-TEP could significantly enhance the recovery of critical-sized bone defects in rats than that of PCL-TEP. To the best of our knowledge, biomimetic PEC-TEP was fabricated and used for the first time to repair bone defects. This novel strategy is promising to fabricate a dECM-based advanced membrane for tissue engineering.
KW - Co-axially structured fibers
KW - Critical-sized bone defect
KW - Decellularized extracellular matrix
KW - Poly(ε-caprolactone)
KW - Tissue-engineered periosteum
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U2 - https://doi.org/10.1016/j.compositesb.2022.109620
DO - https://doi.org/10.1016/j.compositesb.2022.109620
M3 - Article
SN - 1359-8368
VL - 234
SP - 1
EP - 16
JO - Composites Part B: Engineering
JF - Composites Part B: Engineering
M1 - 109620
ER -