Development of severe bronchopulmonary dysplasia is associated with alterations in fecal volatile organic compounds

Daniel J C Berkhout, Hendrik J Niemarkt, Marc A Benninga, Andries E Budding, Anton H van Kaam, Boris W Kramer, Charlene M Pantophlet, Mirjam M van Weissenbruch, Nanne K H de Boer, Tim G J de Meij

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BackgroundThe aim of this study was to evaluate the potential of fecal volatile organic compounds (VOCs), obtained by means of an electronic nose device (Cyranose 320), as early non-invasive biomarker for BPD.MethodsIn this nested case-control study performed at three Neonatal Intensive Care Units, fecal samples obtained at postnatal age of 7, 14, 21, and 28 days from preterm infants with severe bronchopulmonary dysplasia (BPD) were compared with fecal VOC profiles from matched controls. Microbiota analysis was performed by means of IS-pro technique on fecal samples collected at 28 days postnatally.ResultsVOC profiles of infants developing severe BPD (n=15) could be discriminated from matched controls (n=15) at postnatal age of 14 days (area under the curve (±95% confidence interval), P-value, sensitivity, specificity; 0.72 (0.54-0.90), 0.040, 60.0%, 73.3%), 21 days (0.71 (0.52-0.90), 0.049, 66.7%, 73.3%) and 28 days (0.77 (0.59-0.96), 0.017, 69.2%, 69.2%) but not at 7 days. Intestinal microbiota did not differ between BPD subjects and controls.ConclusionFecal VOC profiles of infants developing BPD could be differentiated from controls at postnatal day 14, 21, and 28. VOC differences could not be directed to intestinal microbiota alterations but presumably reflect local and systemic metabolic and inflammatory pathways associated with BPD.Pediatric Research advance online publication, 22 November 2017; doi:10.1038/pr.2017.268.

Original languageEnglish
Pages (from-to)412-419
Number of pages8
JournalPediatric Research
Issue number2
Early online date22 Nov 2017
Publication statusPublished - 1 Feb 2018


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