TY - JOUR
T1 - Diarrhoea in HIV-infected patients
T2 - No evidence of cytokine-mediated inflammation in jejunal mucosa
AU - Snijders, Fransje
AU - Van Deventer, Sander J.H.
AU - Bartelsman, Joep F.W.
AU - Den Otter, Peter
AU - Jansen, Jaap
AU - Mevissen, Marcel L.C.M.
AU - Van Gool, Tom
AU - Danner, Sven A.
AU - Reiss, Peter
PY - 1995/4
Y1 - 1995/4
N2 - Objective: To determine whether a mucosal cytokine-mediated inflammatory response is involved in cryptosporidial or microsporidial diarrhoea, as well as in diarrhoea of unknown origin in HIV-infected patients. Design: Prospective study. Methods: Jejunal biopsies were obtained from HIV-infected patients with diarrhoea. Controls were HIV-infected and HIV-seronegative patients without diarrhoea. Two biopsies were homogenized immediately and two other biopsies were first cultured for 20 h. Cytokines [tumour necrosis factor (TNF), interleukin (lL)-1β, IL-6, IL-8, IL-10], soluble TNF receptors (sTNFR) p55 and p75, and soluble IL-2 receptor (sIL-2R) were assessed in the homogenates and in the supernatants by sandwich enzyme-linked immunosorbent or enzyme-linked binding assays. The cytokine receptors were also measured in serum. Results: Six HIV-infected patients with cryptosporidiosis, six with microsporidiosis, seven with diarrhoea of unknown origin, seven without diarrhoea, and seven HIV-seronegative patients were eligible. Four patients were excluded because of the presence of other pathogens. No cytokines were detected in immediately homogenized jejunal tissue. Following culture, IL-6 and IL-8 levels were higher in HIV-infected patients with diarrhoea of unknown origin than in HIV-seronegative controls without diarrhoea, although this was not statistically significant. No differences in serum or post-culture supernatant sTNFR p55 and p75 levels existed between the HIV-infected patients with or without diarrhoea. sTNFR, IL-1β, IL-10 and the sIL-2R were only detected in low amounts or not at all, and were equally distributed among all patient groups. Conclusions: This study indicates that mucosal cytokine-mediated inflammatory responses do not play an important role in the pathogenesis of different types of diarrhoea in HIV-infected patients. These results do not support the use of immunomodulatory therapy in these patients.
AB - Objective: To determine whether a mucosal cytokine-mediated inflammatory response is involved in cryptosporidial or microsporidial diarrhoea, as well as in diarrhoea of unknown origin in HIV-infected patients. Design: Prospective study. Methods: Jejunal biopsies were obtained from HIV-infected patients with diarrhoea. Controls were HIV-infected and HIV-seronegative patients without diarrhoea. Two biopsies were homogenized immediately and two other biopsies were first cultured for 20 h. Cytokines [tumour necrosis factor (TNF), interleukin (lL)-1β, IL-6, IL-8, IL-10], soluble TNF receptors (sTNFR) p55 and p75, and soluble IL-2 receptor (sIL-2R) were assessed in the homogenates and in the supernatants by sandwich enzyme-linked immunosorbent or enzyme-linked binding assays. The cytokine receptors were also measured in serum. Results: Six HIV-infected patients with cryptosporidiosis, six with microsporidiosis, seven with diarrhoea of unknown origin, seven without diarrhoea, and seven HIV-seronegative patients were eligible. Four patients were excluded because of the presence of other pathogens. No cytokines were detected in immediately homogenized jejunal tissue. Following culture, IL-6 and IL-8 levels were higher in HIV-infected patients with diarrhoea of unknown origin than in HIV-seronegative controls without diarrhoea, although this was not statistically significant. No differences in serum or post-culture supernatant sTNFR p55 and p75 levels existed between the HIV-infected patients with or without diarrhoea. sTNFR, IL-1β, IL-10 and the sIL-2R were only detected in low amounts or not at all, and were equally distributed among all patient groups. Conclusions: This study indicates that mucosal cytokine-mediated inflammatory responses do not play an important role in the pathogenesis of different types of diarrhoea in HIV-infected patients. These results do not support the use of immunomodulatory therapy in these patients.
KW - Cryptosporidiosis
KW - Cytokines
KW - Diarrhoea
KW - HIV
KW - HIV enteropathy
KW - Microsporidiosis
UR - http://www.scopus.com/inward/record.url?scp=0028955191&partnerID=8YFLogxK
U2 - https://doi.org/10.1097/00002030-199504000-00008
DO - https://doi.org/10.1097/00002030-199504000-00008
M3 - Article
C2 - 7794541
SN - 0269-9370
VL - 9
SP - 367
EP - 373
JO - AIDS
JF - AIDS
IS - 4
ER -