Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix

T. D. De Gruijl; H. J. Bontkes; A. J.C. Van den Muysenberg; J. W. Van Oostveen; M. J. Stukart; R. H.M. Verheijen; N. Van der Vange; P. J.F. Snijders; C. J.L.M. Meijer; J. M.M. Walboomers; R. J. Scheper

doi:https://doi.org/10.1016/S0959-8049(98)00371-2

Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix

T. D. De Gruijl, H. J. Bontkes, A. J.C. Van den Muysenberg, J. W. Van Oostveen, M. J. Stukart, R. H.M. Verheijen, N. Van der Vange, P. J.F. Snijders, C. J.L.M. Meijer, J. M.M. Walboomers, R. J. Scheper

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Abstract

The aim of this study was to assess the expression of cytokine transcripts, reflecting the type of ongoing immune responses at the site of human papillomavirus (HPV) infection, in relation to the development of cervical neoplasia. To this end reverse transcription-polymerase chain reaction (RT-PCR) was performed for interferon (IFN)γ, interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12 (p35 and p40), and transforming growth factor (TGFβ1) in snap-frozen cervical biopsies, which were tested for the presence of high risk HPV DNA and histologically classified from normal to invasive carcinoma (n = 40). IFNγ, IL-10 and IL-12 (p35 and p40) transcripts were found to be expressed at significantly lower frequencies in invasive carcinoma as compared with premalignant biopsies (P=0.006, P=0.007 and P=0.002, respectively). IFNγ and IL-10 mRNA were associated with the presence of the IL-12 p35 and p40 transcripts (P=0.008 and P<0.00001, respectively). These results were consistent with a locally reduced cellular (type 1) immunity correlating with HPV-induced invasive cervical carcinoma.

Original language	English
Pages (from-to)	490-497
Number of pages	8
Journal	European Journal of Cancer
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/S0959-8049(98)00371-2
Publication status	Published - 1 Jan 1999

Keywords

Cervical neoplasia
Cytokines
Human papillomavirus
IFNγ
IL- 12
IL-10
RT-PCR
T cell

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De Gruijl, T. D., Bontkes, H. J., Van den Muysenberg, A. J. C., Van Oostveen, J. W., Stukart, M. J., Verheijen, R. H. M., Van der Vange, N., Snijders, P. J. F., Meijer, C. J. L. M., Walboomers, J. M. M., & Scheper, R. J. (1999). Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix. European Journal of Cancer, 35(3), 490-497. https://doi.org/10.1016/S0959-8049(98)00371-2

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title = "Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix",

abstract = "The aim of this study was to assess the expression of cytokine transcripts, reflecting the type of ongoing immune responses at the site of human papillomavirus (HPV) infection, in relation to the development of cervical neoplasia. To this end reverse transcription-polymerase chain reaction (RT-PCR) was performed for interferon (IFN)γ, interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12 (p35 and p40), and transforming growth factor (TGFβ1) in snap-frozen cervical biopsies, which were tested for the presence of high risk HPV DNA and histologically classified from normal to invasive carcinoma (n = 40). IFNγ, IL-10 and IL-12 (p35 and p40) transcripts were found to be expressed at significantly lower frequencies in invasive carcinoma as compared with premalignant biopsies (P=0.006, P=0.007 and P=0.002, respectively). IFNγ and IL-10 mRNA were associated with the presence of the IL-12 p35 and p40 transcripts (P=0.008 and P<0.00001, respectively). These results were consistent with a locally reduced cellular (type 1) immunity correlating with HPV-induced invasive cervical carcinoma.",

keywords = "Cervical neoplasia, Cytokines, Human papillomavirus, IFNγ, IL- 12, IL-10, RT-PCR, T cell",

author = "{De Gruijl}, {T. D.} and Bontkes, {H. J.} and {Van den Muysenberg}, {A. J.C.} and {Van Oostveen}, {J. W.} and Stukart, {M. J.} and Verheijen, {R. H.M.} and {Van der Vange}, N. and Snijders, {P. J.F.} and Meijer, {C. J.L.M.} and Walboomers, {J. M.M.} and Scheper, {R. J.}",

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De Gruijl, TD , Bontkes, HJ, Van den Muysenberg, AJC, Van Oostveen, JW, Stukart, MJ, Verheijen, RHM, Van der Vange, N, Snijders, PJF, Meijer, CJLM, Walboomers, JMM & Scheper, RJ 1999, 'Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix', European Journal of Cancer, vol. 35, no. 3, pp. 490-497. https://doi.org/10.1016/S0959-8049(98)00371-2

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T1 - Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix

AU - De Gruijl, T. D.

AU - Bontkes, H. J.

AU - Van den Muysenberg, A. J.C.

AU - Van Oostveen, J. W.

AU - Stukart, M. J.

AU - Verheijen, R. H.M.

AU - Van der Vange, N.

AU - Snijders, P. J.F.

AU - Meijer, C. J.L.M.

AU - Walboomers, J. M.M.

AU - Scheper, R. J.

PY - 1999/1/1

Y1 - 1999/1/1

N2 - The aim of this study was to assess the expression of cytokine transcripts, reflecting the type of ongoing immune responses at the site of human papillomavirus (HPV) infection, in relation to the development of cervical neoplasia. To this end reverse transcription-polymerase chain reaction (RT-PCR) was performed for interferon (IFN)γ, interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12 (p35 and p40), and transforming growth factor (TGFβ1) in snap-frozen cervical biopsies, which were tested for the presence of high risk HPV DNA and histologically classified from normal to invasive carcinoma (n = 40). IFNγ, IL-10 and IL-12 (p35 and p40) transcripts were found to be expressed at significantly lower frequencies in invasive carcinoma as compared with premalignant biopsies (P=0.006, P=0.007 and P=0.002, respectively). IFNγ and IL-10 mRNA were associated with the presence of the IL-12 p35 and p40 transcripts (P=0.008 and P<0.00001, respectively). These results were consistent with a locally reduced cellular (type 1) immunity correlating with HPV-induced invasive cervical carcinoma.

AB - The aim of this study was to assess the expression of cytokine transcripts, reflecting the type of ongoing immune responses at the site of human papillomavirus (HPV) infection, in relation to the development of cervical neoplasia. To this end reverse transcription-polymerase chain reaction (RT-PCR) was performed for interferon (IFN)γ, interleukin (IL)-2, IL-4, IL-5, IL-10, IL-12 (p35 and p40), and transforming growth factor (TGFβ1) in snap-frozen cervical biopsies, which were tested for the presence of high risk HPV DNA and histologically classified from normal to invasive carcinoma (n = 40). IFNγ, IL-10 and IL-12 (p35 and p40) transcripts were found to be expressed at significantly lower frequencies in invasive carcinoma as compared with premalignant biopsies (P=0.006, P=0.007 and P=0.002, respectively). IFNγ and IL-10 mRNA were associated with the presence of the IL-12 p35 and p40 transcripts (P=0.008 and P<0.00001, respectively). These results were consistent with a locally reduced cellular (type 1) immunity correlating with HPV-induced invasive cervical carcinoma.

KW - Cervical neoplasia

KW - Cytokines

KW - Human papillomavirus

KW - IFNγ

KW - IL- 12

KW - IL-10

KW - RT-PCR

KW - T cell

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DO - https://doi.org/10.1016/S0959-8049(98)00371-2

M3 - Article

C2 - 10448305

SN - 0959-8049

VL - 35

SP - 490

EP - 497

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 3

ER -

Differences in cytokine mRNA profiles between premalignant and malignant lesions of the uterine cervix

Abstract

Keywords

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