Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display

C. Willemien Van Der Houven Van Oordt; Theo G. Schouten; Alex J. Van Der Eb; Marco L. Breuer

doi:https://doi.org/10.1002/(SICI)1098-2744(199901)24:1<29::AID-MC5>3.0.CO;2-L

Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display

C. Willemien Van Der Houven Van Oordt, Theo G. Schouten, Alex J. Van Der Eb, Marco L. Breuer

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Eμ-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro-element that was significantly overexpressed in a subset of lymphomas as compared with non- lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.

Original language	English
Pages (from-to)	29-35
Number of pages	7
Journal	Molecular carcinogenesis
Volume	24
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1098-2744(199901)24:1<29::AID-MC5>3.0.CO;2-L
Publication status	Published - 1999
Externally published	Yes

Keywords

Cytotoxic T-lymphocyte lipase
Eμ-pim-1 mice
Glutathione S-transferase
VL30 retro-element
X irradiation

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title = "Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display",

abstract = "Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Eμ-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro-element that was significantly overexpressed in a subset of lymphomas as compared with non- lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.",

keywords = "Cytotoxic T-lymphocyte lipase, Eμ-pim-1 mice, Glutathione S-transferase, VL30 retro-element, X irradiation",

author = "{Van Der Houven Van Oordt}, {C. Willemien} and Schouten, {Theo G.} and {Van Der Eb}, {Alex J.} and Breuer, {Marco L.}",

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T1 - Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display

AU - Van Der Houven Van Oordt, C. Willemien

AU - Schouten, Theo G.

AU - Van Der Eb, Alex J.

AU - Breuer, Marco L.

PY - 1999

Y1 - 1999

N2 - Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Eμ-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro-element that was significantly overexpressed in a subset of lymphomas as compared with non- lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.

AB - Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Eμ-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro-element that was significantly overexpressed in a subset of lymphomas as compared with non- lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.

KW - Cytotoxic T-lymphocyte lipase

KW - Eμ-pim-1 mice

KW - Glutathione S-transferase

KW - VL30 retro-element

KW - X irradiation

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M3 - Article

C2 - 10029408

SN - 0899-1987

VL - 24

SP - 29

EP - 35

JO - Molecular carcinogenesis

JF - Molecular carcinogenesis

IS - 1

ER -

Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display

Abstract

Keywords

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