Disturbance of Microbial Core Species in New-Onset Juvenile Idiopathic Arthritis

Petra Hissink Muller, Tim G. J. de Meij, Michiel Westedt, Evelien F. J. de Groot, Cornelia F. Allaart, Danielle M. C. Brinkman, Dieneke Schonenberg-Meinema, Merlijn van den Berg, Lisette W. A. van Suijlekom-Smit, Marion van Rossum, Andries E. Budding, Rebecca ten Cate

Research output: Contribution to JournalArticleAcademicpeer-review

10 Citations (Scopus)


Over the past decades, the intestinal microbiota has increasingly gained attention in studies addressing the pathophysiology of (pediatric) autoimmune diseases, including inflammatory joint diseases, inflammatory bowel disease (IBD), and type 1 diabetes. In this study, we have analyzed the composition of gut microbiota of newly diagnosed juvenile idiopathic arthritis (JIA) patients, prior to initiation of disease-modifying antirheumatic drugs (DMARDs). Fecal microbiota profiles of 8 JIA patients (median age: 11.1 years, 6 girls) were compared with 22 healthy age-matched controls using IS-pro, a 16S-23S interspacer (IS) region-based, eubacterial molecular detection technique. By partial least squares discriminant analysis (PLS-DA), microbiota profiles of JIA and controls could significantly be discriminated based on a limited set of species belonging to the phylum Bacteroidetes ((sic)Fig. 2), but not within other phyla, with a sensitivity of 88%, specificity of 73% ((sic)Fig. 3), and area under the curve (AUC) 0.87 (95% CI: 0.73-0.87). These discriminative species have been considered to be part of the microbial core in healthy children. Conclusion Our findings add to the increasing notion that the gut microbiota may be involved in the pathophysiology of JIA. Species involved in the discrimination between JIA and controls are members of the microbial core in the healthy state. Expanding knowledge on JIA-specificmicrobial signatures and host interactions may open avenues to explore options to develop individualized, microbiota-based preventive, and therapeutic interventions in JIA
Original languageEnglish
Pages (from-to)131-135
JournalJournal of Pediatric Infectious Diseases
Issue number2
Publication statusPublished - Jun 2017


  • IS-pro
  • autoimmunity
  • gut
  • intestinal microbiota
  • juvenile idiopathic arthritis

Cite this