TY - JOUR
T1 - DNA double-strand break repair in parental chromatin of mouse zygotes, the first cell cycle as an origin of de novo mutation
AU - Derijck, Alwin
AU - Van der heijden, Godfried
AU - Giele, Maud
AU - Philippens, Marielle
AU - De boer, Peter
PY - 2008/7
Y1 - 2008/7
N2 - In the human, the contribution of the sexes to the genetic load is dissimilar. Especially for point mutations, expanded simple tandem repeats and structural chromosome mutations, the contribution of the male germline is dominant. Far less is known about the male germ cell stage(s) that are most vulnerable to mutation contraction. For the understanding of de novo mutation induction in the germline, mechanistic insight of DNA repair in the zygote is mandatory. At the onset of embryonic development, the parental chromatin sets occupy one pronucleus (PN) each and DNA repair can be regarded as a maternal trait, depending on proteins and mRNAs provided by the oocyte. Repair of DNA double-strand breaks (DSBs) is executed by non-homologous end joining (NHEJ) and homologous recombination (HR). Differentiated somatic cells often resolve DSBs by NHEJ, whereas embryonic stem cells preferably use HR. We show NHEJ and HR to be both functional during the zygotic cell cycle. NHEJ is already active during replacement of sperm protamines by nucleosomes. The kinetics of G1 repair is influenced by DNA-PKcs hypomorphic activity. Both HR and NHEJ are operative in S-phase, HR being more active in the male PN. DNA-PKcs deficiency upregulates the HR activity. Both after sperm remodeling and at first mitosis, spontaneous levels of γH2AX foci (marker for DSBs) are high. All immunoflurescent indices of DNA damage and DNA repair point at greater spontaneous damage and induced repair activity in paternal chromatin in the zygote.
AB - In the human, the contribution of the sexes to the genetic load is dissimilar. Especially for point mutations, expanded simple tandem repeats and structural chromosome mutations, the contribution of the male germline is dominant. Far less is known about the male germ cell stage(s) that are most vulnerable to mutation contraction. For the understanding of de novo mutation induction in the germline, mechanistic insight of DNA repair in the zygote is mandatory. At the onset of embryonic development, the parental chromatin sets occupy one pronucleus (PN) each and DNA repair can be regarded as a maternal trait, depending on proteins and mRNAs provided by the oocyte. Repair of DNA double-strand breaks (DSBs) is executed by non-homologous end joining (NHEJ) and homologous recombination (HR). Differentiated somatic cells often resolve DSBs by NHEJ, whereas embryonic stem cells preferably use HR. We show NHEJ and HR to be both functional during the zygotic cell cycle. NHEJ is already active during replacement of sperm protamines by nucleosomes. The kinetics of G1 repair is influenced by DNA-PKcs hypomorphic activity. Both HR and NHEJ are operative in S-phase, HR being more active in the male PN. DNA-PKcs deficiency upregulates the HR activity. Both after sperm remodeling and at first mitosis, spontaneous levels of γH2AX foci (marker for DSBs) are high. All immunoflurescent indices of DNA damage and DNA repair point at greater spontaneous damage and induced repair activity in paternal chromatin in the zygote.
UR - http://www.scopus.com/inward/record.url?scp=45749083393&partnerID=8YFLogxK
U2 - https://doi.org/10.1093/hmg/ddn090
DO - https://doi.org/10.1093/hmg/ddn090
M3 - Article
C2 - 18353795
SN - 0964-6906
VL - 17
SP - 1922
EP - 1937
JO - Human Molecular Genetics
JF - Human Molecular Genetics
IS - 13
ER -