TY - JOUR
T1 - Donor variation in stored platelets
T2 - Higher metabolic rates of platelets are associated with mean platelet volume, activation and donor health
AU - Bontekoe, Ido J.
AU - van der Meer, Pieter F.
AU - Tanis, Bea C.
AU - de Korte, Dirk
AU - Verhoeven, Arthur J.
AU - Raat, Nicolaas J. H.
AU - Specht, Patricia A. C.
AU - Mik, Egbert G.
AU - Klei, Thomas R. L.
N1 - Funding Information: We thank Rob Hoenderdaal and Davina Ardanary for technical assistance. We are also grateful to Femmeke Prinsze and Rebecca Broekman-Piets for donor information and to Femke Schrauwen of the Amsterdam UMCs for analysis of all lipid profiles. Publisher Copyright: © 2022 AABB.
PY - 2022/12
Y1 - 2022/12
N2 - Background: Platelets (PLTs) differ in glycolytic activity, resulting in rapid acidification of ‘poor’ storing PLT concentrates (PCs) in plasma, or depletion of glucose when stored in PLT additive solution (PAS). We aimed to understand why PLT glycolysis rates vary between donors and how this affects storage performance. Study Design and Methods: Buffy coats from donors <45, 45–70 and >70 years were selected and single-donor PCs in plasma or PAS-E were prepared. PCs were stored for 8 days at 22 ± 2°C and sampled regularly for analysis. Mitochondrial activity was analyzed with an Oroboros oxygraph. Age groups, or subgroups divided into quartiles based on glucose consumption, were analyzed with ANOVA. Results: In each comparison, PCs of the different groups were not different in volume and cellular composition. PLTs with the highest glucose consumption had a higher initial mean platelet volume (MPV) and developed higher CD62P expression and Annexin A5 binding during storage. Higher glycolytic activity in these PLTs was not a compensation for lower mitochondrial ATP production, because mitochondrial ATP-linked respiration of fresh PLTs correlated positively with MPV (R2 = 0.71). Donors of high glucose-consuming PLTs had more health-related issues. Storage properties of PCs from donors over 70 were not significantly different compared to PCs from donors younger than 45 years. Conclusions: High glucose-consuming PCs developing higher activation levels, not only displayed enhanced mitochondrial activity but were also found to contain larger PLTs, as determined by MPV. Storage performance of PLTs was found to be associated with donor health, but not with donor age.
AB - Background: Platelets (PLTs) differ in glycolytic activity, resulting in rapid acidification of ‘poor’ storing PLT concentrates (PCs) in plasma, or depletion of glucose when stored in PLT additive solution (PAS). We aimed to understand why PLT glycolysis rates vary between donors and how this affects storage performance. Study Design and Methods: Buffy coats from donors <45, 45–70 and >70 years were selected and single-donor PCs in plasma or PAS-E were prepared. PCs were stored for 8 days at 22 ± 2°C and sampled regularly for analysis. Mitochondrial activity was analyzed with an Oroboros oxygraph. Age groups, or subgroups divided into quartiles based on glucose consumption, were analyzed with ANOVA. Results: In each comparison, PCs of the different groups were not different in volume and cellular composition. PLTs with the highest glucose consumption had a higher initial mean platelet volume (MPV) and developed higher CD62P expression and Annexin A5 binding during storage. Higher glycolytic activity in these PLTs was not a compensation for lower mitochondrial ATP production, because mitochondrial ATP-linked respiration of fresh PLTs correlated positively with MPV (R2 = 0.71). Donors of high glucose-consuming PLTs had more health-related issues. Storage properties of PCs from donors over 70 were not significantly different compared to PCs from donors younger than 45 years. Conclusions: High glucose-consuming PCs developing higher activation levels, not only displayed enhanced mitochondrial activity but were also found to contain larger PLTs, as determined by MPV. Storage performance of PLTs was found to be associated with donor health, but not with donor age.
KW - blood component preparations
KW - donors
KW - platelet transfusion
UR - http://www.scopus.com/inward/record.url?scp=85140369876&partnerID=8YFLogxK
U2 - https://doi.org/10.1111/trf.17160
DO - https://doi.org/10.1111/trf.17160
M3 - Article
C2 - 36278429
SN - 0041-1132
VL - 62
SP - 2609
EP - 2620
JO - Transfusion
JF - Transfusion
IS - 12
ER -