E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements

G. Bain; E. C. Maandag; D. J. Izon; D. Amsen; A. M. Kruisbeek; B. C. Weintraub; I. Krop; M. S. Schlissel; A. J. Feeney; M. van Roon

doi:https://doi.org/10.1016/0092-8674(94)90077-9

E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements

G. Bain, E. C. Maandag, D. J. Izon, D. Amsen, A. M. Kruisbeek, B. C. Weintraub, I. Krop, M. S. Schlissel, A. J. Feeney, M. van Roon

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Abstract

E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation

Original language	English
Pages (from-to)	885-892
Journal	Cell
Volume	79
Issue number	5
DOIs	https://doi.org/10.1016/0092-8674(94)90077-9
Publication status	Published - 1994

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https://doi.org/10.1016/0092-8674(94)90077-9

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@article{aa16c6aeb8464eacaec33336c7255321,

title = "E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements",

abstract = "E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation",

author = "G. Bain and Maandag, {E. C.} and Izon, {D. J.} and D. Amsen and Kruisbeek, {A. M.} and Weintraub, {B. C.} and I. Krop and Schlissel, {M. S.} and Feeney, {A. J.} and {van Roon}, M.",

year = "1994",

doi = "https://doi.org/10.1016/0092-8674(94)90077-9",

language = "English",

volume = "79",

pages = "885--892",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - E2A proteins are required for proper B cell development and initiation of immunoglobulin gene rearrangements

AU - Bain, G.

AU - Maandag, E. C.

AU - Izon, D. J.

AU - Amsen, D.

AU - Kruisbeek, A. M.

AU - Weintraub, B. C.

AU - Krop, I.

AU - Schlissel, M. S.

AU - Feeney, A. J.

AU - van Roon, M.

PY - 1994

Y1 - 1994

N2 - E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation

AB - E12 and E47 are two helix-loop-helix transcription factors that arise by alternative splicing of the E2A gene. Both have been implicated in the regulation of immunoglobulin gene expression. We have now generated E2A (-/-) mice by gene targeting. E2A-null mutant mice fail to generate mature B cells. The arrest of B cell development occurs at an early stage, since no immunoglobulin DJ rearrangements can be detected in homozygous mutant mice. While immunoglobulin germline I mu RAG-1, mb-1, CD19, and lambda 5 transcripts are dramatically reduced in fetal livers of E2A (-/-) mice, B29 and mu degrees transcripts are present, but at lower levels. In addition, we show that Pax-5 transcripts are significantly reduced in fetal livers of E2A (-/-) mice. These data suggest a crucial role for E2A products as central regulators in early B cell differentiation

U2 - https://doi.org/10.1016/0092-8674(94)90077-9

DO - https://doi.org/10.1016/0092-8674(94)90077-9

M3 - Article

C2 - 8001125

SN - 0092-8674

VL - 79

SP - 885

EP - 892

JO - Cell

JF - Cell

IS - 5

ER -