TY - JOUR
T1 - Early myocardial dysfunction is not caused by mitochondrial abnormalities in a rat model of peritonitis
AU - Smeding, Lonneke
AU - van der Laarse, Willem J.
AU - van Veelen, Toke A.
AU - Lamberts, Regis R.
AU - Niessen, Hans W. M.
AU - Kneyber, Martin C. J.
AU - Johan Groeneveld, A. B.
AU - Plötz, Frans B.
AU - Kneijber, M.C.J.
PY - 2012/7
Y1 - 2012/7
N2 - Background: Patients with complicated intra-abdominal infections are prone to develop multiple organ failure, including myocardial dysfunction. We hypothesized that early dysfunction during sepsis is associated with inflammation, mitochondrial injury, impaired mitochondrial function, and activation of mitochondrial biogenesis. Materials and Methods: Rats received lipopolysaccharide (LPS, n = 11) intraperitoneally. Healthy rats (n = 6) served as controls. Myocardial function was measured ex vivo in an isolated Langendorff-perfused heart set-up. Myocardial vascular cell adhesion molecule-1 (VCAM-1) expression was determined by immunofluorescence microscopy. Cytochrome c release and cytochrome c oxidase (COX IV) activity were measured by immunohistochemistry and enzyme histochemistry, respectively. Protein expression of tumor necrosis factor-α (TNF-α), B-cell lymphoma (Bcl)-2, peroxisome proliferator activated receptor γ cofactor 1α (PGC-1α), and mitochondrial transcription factor A (TFAM) were analyzed by Western blot technique. Mitochondria were studied by electron microscopy. Results: Two hours after LPS injection, developed pressure had decreased and after 4 h myocardial contractility (+dP/dt) and relaxation (-dP/dt) also had decreased. TNF-α protein expression was increased after 2 h and returned to normal at 4 h, whereas after 4 h VCAM-1 expression was higher in LPS-treated animals. At 2 h a substrate-dependent increase in COXIV-activity was seen, but no mitochondrial damage occurred as cytochrome c release, COX IV activity and Bcl-2, PGC-1α or TFAM expression were not changed. Electron microscopy did not reveal differences in myocardial mitochondrial characteristics between LPS-treated and control rats. Conclusions: Early myocardial dysfunction in sepsis is associated with myocardial inflammation but not with mitochondrial injury, impaired mitochondrial function, or activated mitochondrial biogenesis. © 2012 Elsevier Inc. All rights reserved.
AB - Background: Patients with complicated intra-abdominal infections are prone to develop multiple organ failure, including myocardial dysfunction. We hypothesized that early dysfunction during sepsis is associated with inflammation, mitochondrial injury, impaired mitochondrial function, and activation of mitochondrial biogenesis. Materials and Methods: Rats received lipopolysaccharide (LPS, n = 11) intraperitoneally. Healthy rats (n = 6) served as controls. Myocardial function was measured ex vivo in an isolated Langendorff-perfused heart set-up. Myocardial vascular cell adhesion molecule-1 (VCAM-1) expression was determined by immunofluorescence microscopy. Cytochrome c release and cytochrome c oxidase (COX IV) activity were measured by immunohistochemistry and enzyme histochemistry, respectively. Protein expression of tumor necrosis factor-α (TNF-α), B-cell lymphoma (Bcl)-2, peroxisome proliferator activated receptor γ cofactor 1α (PGC-1α), and mitochondrial transcription factor A (TFAM) were analyzed by Western blot technique. Mitochondria were studied by electron microscopy. Results: Two hours after LPS injection, developed pressure had decreased and after 4 h myocardial contractility (+dP/dt) and relaxation (-dP/dt) also had decreased. TNF-α protein expression was increased after 2 h and returned to normal at 4 h, whereas after 4 h VCAM-1 expression was higher in LPS-treated animals. At 2 h a substrate-dependent increase in COXIV-activity was seen, but no mitochondrial damage occurred as cytochrome c release, COX IV activity and Bcl-2, PGC-1α or TFAM expression were not changed. Electron microscopy did not reveal differences in myocardial mitochondrial characteristics between LPS-treated and control rats. Conclusions: Early myocardial dysfunction in sepsis is associated with myocardial inflammation but not with mitochondrial injury, impaired mitochondrial function, or activated mitochondrial biogenesis. © 2012 Elsevier Inc. All rights reserved.
KW - Animals
KW - Disease Models, Animal
KW - Heart
KW - Journal Article
KW - Lipopolysaccharides
KW - Male
KW - Mitochondria, Heart
KW - Myocardial Contraction
KW - Myocardium
KW - Peritonitis
KW - Rats
KW - Rats, Wistar
KW - Time Factors
KW - Tumor Necrosis Factor-alpha
KW - Vascular Cell Adhesion Molecule-1
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84862010229&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/21816428
U2 - https://doi.org/10.1016/j.jss.2011.05.055
DO - https://doi.org/10.1016/j.jss.2011.05.055
M3 - Article
C2 - 21816428
SN - 0022-4804
VL - 176
SP - 178
EP - 184
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -