TY - JOUR
T1 - ED2-positive perivascular phagocytes produce interleukin-1β during delayed neuronal loss in the facial nucleus of the rat
AU - Angelov, D. N.
AU - Walther, M.
AU - Streppel, M.
AU - Guntinas-Lichius, O.
AU - Van Dam, A. M.
AU - Stennert, E.
AU - Neiss, W. F.
PY - 1998/12/15
Y1 - 1998/12/15
N2 - Injection of Fluoro-Gold (FG) into the whisker pad of rats yields stable retrograde labeling of facial motoneurons. Subsequent removal of 10 mm from all facial nerve branches permanently deprives the FG-labeled motoneurons from their targets and the motoneurons gradually die. Neuronal debris is phagocytized by two types of neuronophages: parenchymal microglia (monoclonal antibody [MAb] OX42-positive, MAb ED2-negative) and perivascular phagocytes (OX42-negative, ED2-positive). Because both types of neuronophages express major histocompatibility complex (MHC) class II glycoproteins (MAb OX6- positive), they are considered to be the potential antigen-presenting cells of the brain. To check this hypothesis, we tested whether both types of neuronophages also synthetize the co-stimulatory cytokine interleukin-1β (IL-1β) immunocytochemically visualized by MAbs SILK-5/6. Employing combined fluorescent visualization of antigens (OX6, ED2, and SILK-5/6) in sections containing fluorescent (FG-prelabeled) neuronophages, we found that, during slowly occurring neuronal loss, the vast majority of IL-1β immunoreactive neuronophages were of perivascular (ED2-positive) origin. We concluded that, during delayed neuronal death 'behind' an intact blood-brain barrier, the perivascular phagocytes were more likely to function as antigen-presenting cells than the parenchymal microglia.
AB - Injection of Fluoro-Gold (FG) into the whisker pad of rats yields stable retrograde labeling of facial motoneurons. Subsequent removal of 10 mm from all facial nerve branches permanently deprives the FG-labeled motoneurons from their targets and the motoneurons gradually die. Neuronal debris is phagocytized by two types of neuronophages: parenchymal microglia (monoclonal antibody [MAb] OX42-positive, MAb ED2-negative) and perivascular phagocytes (OX42-negative, ED2-positive). Because both types of neuronophages express major histocompatibility complex (MHC) class II glycoproteins (MAb OX6- positive), they are considered to be the potential antigen-presenting cells of the brain. To check this hypothesis, we tested whether both types of neuronophages also synthetize the co-stimulatory cytokine interleukin-1β (IL-1β) immunocytochemically visualized by MAbs SILK-5/6. Employing combined fluorescent visualization of antigens (OX6, ED2, and SILK-5/6) in sections containing fluorescent (FG-prelabeled) neuronophages, we found that, during slowly occurring neuronal loss, the vast majority of IL-1β immunoreactive neuronophages were of perivascular (ED2-positive) origin. We concluded that, during delayed neuronal death 'behind' an intact blood-brain barrier, the perivascular phagocytes were more likely to function as antigen-presenting cells than the parenchymal microglia.
KW - Antigen presentation
KW - Motoneuron
KW - Neuronophagia
KW - Perivascular cells
UR - http://www.scopus.com/inward/record.url?scp=0032534701&partnerID=8YFLogxK
U2 - https://doi.org/10.1002/(SICI)1097-4547(19981215)54:6<820::AID-JNR10>3.0.CO;2-X
DO - https://doi.org/10.1002/(SICI)1097-4547(19981215)54:6<820::AID-JNR10>3.0.CO;2-X
M3 - Article
C2 - 9856866
SN - 0360-4012
VL - 54
SP - 820
EP - 827
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 6
ER -