TY - JOUR
T1 - Effect of albumin on the photodynamic inactivation of microorganisms by a cationic porphyrin
AU - Lambrechts, Saskia A. G.
AU - Aalders, Maurice C. G.
AU - Verbraak, Frank D.
AU - Lagerberg, Johan W. M.
AU - Dankert, Jacob B.
AU - Schuitmaker, Johannes J.
PY - 2005
Y1 - 2005
N2 - Background: Photodynamic inactivation (PDI) employs visible light and a photosensitizer to inactivate cells. The technique is currently clinically used for the treatment of several malignancies. However, the PDI of microorganisms still remains in the research phase. Purpose: To study the effect of human blood plasma and human serum albumin (HSA) on the PDI of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Methods: PDI experiments were performed using white light (30 mW cm(-2)) and the cationic 5-phenyl- 10, 1 5,20-tris(N-methyl-4pyridyl)porphyrin chloride (TriP[4]) as photo sensitizer. Results: The microorganisms could be successfully photoinactivated by TriP[4] when suspended in phosphate buffered saline (PBS). In this medium, P. aeruginosa was the most resistant microorganism. Changing the suspending medium from PBS to human blood plasma reduced the PDI of all three microorganisms. In human blood plasma C. albicans was the most resistant microorganism. The same results were obtained with 4.5% and 7% HSA/PBS suspensions. Conclusions: Albumin inhibits the PDI of S. aureus, P. aeruginosa and C. albicans in a dose dependent manner. However, our results are encouraging towards the potential future application of PDI for the treatment of superficial wound infections caused by S. aureus, P. aeruginosa and C. albicans. (c) 2004 Elsevier B.V. All rights reserved
AB - Background: Photodynamic inactivation (PDI) employs visible light and a photosensitizer to inactivate cells. The technique is currently clinically used for the treatment of several malignancies. However, the PDI of microorganisms still remains in the research phase. Purpose: To study the effect of human blood plasma and human serum albumin (HSA) on the PDI of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Methods: PDI experiments were performed using white light (30 mW cm(-2)) and the cationic 5-phenyl- 10, 1 5,20-tris(N-methyl-4pyridyl)porphyrin chloride (TriP[4]) as photo sensitizer. Results: The microorganisms could be successfully photoinactivated by TriP[4] when suspended in phosphate buffered saline (PBS). In this medium, P. aeruginosa was the most resistant microorganism. Changing the suspending medium from PBS to human blood plasma reduced the PDI of all three microorganisms. In human blood plasma C. albicans was the most resistant microorganism. The same results were obtained with 4.5% and 7% HSA/PBS suspensions. Conclusions: Albumin inhibits the PDI of S. aureus, P. aeruginosa and C. albicans in a dose dependent manner. However, our results are encouraging towards the potential future application of PDI for the treatment of superficial wound infections caused by S. aureus, P. aeruginosa and C. albicans. (c) 2004 Elsevier B.V. All rights reserved
U2 - https://doi.org/10.1016/j.jphotobiol.2004.11.020
DO - https://doi.org/10.1016/j.jphotobiol.2004.11.020
M3 - Article
C2 - 15792879
SN - 1011-1344
VL - 79
SP - 51
EP - 57
JO - Journal of photochemistry and photobiology. B, Biology
JF - Journal of photochemistry and photobiology. B, Biology
IS - 1
ER -