TY - JOUR
T1 - Effect of antioxidants on hyperoxia-induced chromosomal breakage in Chinese hamster ovary cells
T2 - Protection by carnosine
AU - Gille, J. J.P.
AU - Pasman, P.
AU - Van Berkel, C. G.M.
AU - Joenje, H.
N1 - Funding Information: The authors wish to thank Professor A.Bast for his help with synthesizing the giutathione monoethylester. This study was supported by the Dutch Cancer Society (Nederlandse Kankerbestrijding).
PY - 1991/7
Y1 - 1991/7
N2 - We have studied the effect of various compounds, known as antioxidants, on the level of hyperoxia (80-90% O2)-induced chromosomal aberrations in Chinese hamster ovary cells: ascorbic acid, α-tocopherol, carnosine, imidazole-4-acetic acid, glutathione monoethylester, N-acetylcysteine and ethoxyquin. Carnosine (β-alanyl-histidine) appeared to be the only compound that reduced chromosomal breakage. The effect was also present in cultures post-treated with caffeine (at 2.5 mM, 3 h before harvest), indicating that the apparent protection was not due to selective arrest of chromosomally damaged cells in the G2 phase of the cell cycle. Imidazole-4-acetic acid, a compound structurally very similar to carnosine, had no detectable effect. Ascorbic acid, N-acetycysteine, glutathione monoethylester and ethoxyquin were found to have a pro-oxidant effect, i.e. they apparently potentiated the clastogenic effect of hyperoxia. Carnosine is the first compound shown to protect against the clastogenicity of normobaric hyperoxia and may thus be a useful tool in elucidating the underlying mechanism.
AB - We have studied the effect of various compounds, known as antioxidants, on the level of hyperoxia (80-90% O2)-induced chromosomal aberrations in Chinese hamster ovary cells: ascorbic acid, α-tocopherol, carnosine, imidazole-4-acetic acid, glutathione monoethylester, N-acetylcysteine and ethoxyquin. Carnosine (β-alanyl-histidine) appeared to be the only compound that reduced chromosomal breakage. The effect was also present in cultures post-treated with caffeine (at 2.5 mM, 3 h before harvest), indicating that the apparent protection was not due to selective arrest of chromosomally damaged cells in the G2 phase of the cell cycle. Imidazole-4-acetic acid, a compound structurally very similar to carnosine, had no detectable effect. Ascorbic acid, N-acetycysteine, glutathione monoethylester and ethoxyquin were found to have a pro-oxidant effect, i.e. they apparently potentiated the clastogenic effect of hyperoxia. Carnosine is the first compound shown to protect against the clastogenicity of normobaric hyperoxia and may thus be a useful tool in elucidating the underlying mechanism.
UR - http://www.scopus.com/inward/record.url?scp=0025837512&partnerID=8YFLogxK
U2 - https://doi.org/10.1093/mutage/6.4.313
DO - https://doi.org/10.1093/mutage/6.4.313
M3 - Article
C2 - 1943722
SN - 0267-8357
VL - 6
SP - 313
EP - 318
JO - Mutagenesis
JF - Mutagenesis
IS - 4
ER -