Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract

Boudewijn J.M. Braakhuis; Marij J.P. Welters; Jacqueline Cloos; Jeannette E. Pankras; Serge J. Smeets; Anne Marie J. Fichtinger-Schepman

doi:https://doi.org/10.1016/S0304-3835(99)00016-6

Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract

Boudewijn J.M. Braakhuis, Marij J.P. Welters, Jacqueline Cloos, Jeannette E. Pankras, Serge J. Smeets, Anne Marie J. Fichtinger-Schepman

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	217-220
Number of pages	4
Journal	Cancer Letters
Volume	138
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(99)00016-6
Publication status	Published - 26 Apr 1999

Keywords

Cisplatin
Head and neck
Keratinocytes
Non-malignant cells
Pt accumulation
Squamous cells

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Braakhuis, B. J. M., Welters, M. J. P., Cloos, J., Pankras, J. E., Smeets, S. J., & Fichtinger-Schepman, A. M. J. (1999). Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract. Cancer Letters, 138(1-2), 217-220. https://doi.org/10.1016/S0304-3835(99)00016-6

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title = "Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract",

abstract = "The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.",

keywords = "Cisplatin, Head and neck, Keratinocytes, Non-malignant cells, Pt accumulation, Squamous cells",

author = "Braakhuis, {Boudewijn J.M.} and Welters, {Marij J.P.} and Jacqueline Cloos and Pankras, {Jeannette E.} and Smeets, {Serge J.} and Fichtinger-Schepman, {Anne Marie J.}",

year = "1999",

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Braakhuis, BJM, Welters, MJP, Cloos, J, Pankras, JE, Smeets, SJ & Fichtinger-Schepman, AMJ 1999, 'Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract', Cancer Letters, vol. 138, no. 1-2, pp. 217-220. https://doi.org/10.1016/S0304-3835(99)00016-6

Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract. / Braakhuis, Boudewijn J.M.; Welters, Marij J.P.; Cloos, Jacqueline et al.
In: Cancer Letters, Vol. 138, No. 1-2, 26.04.1999, p. 217-220.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract

AU - Braakhuis, Boudewijn J.M.

AU - Welters, Marij J.P.

AU - Cloos, Jacqueline

AU - Pankras, Jeannette E.

AU - Smeets, Serge J.

AU - Fichtinger-Schepman, Anne Marie J.

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N2 - The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.

AB - The aim of the present study was to investigate how normal head and neck epithelial cells (NHNEC) respond to cisplatin compared to their neoplastic counterparts with respect to intracellular platinum (Pt) levels and growth inhibition. A colorimetric assay was used to assess growth inhibition after exposure to cisplatin for 72 h. Growth inhibition did not differ between cultures of neoplastic (n=5) and normal cells (n=5). Intracellular Pt levels, determined with atomic absorption spectroscopy were about 30-fold higher in the normal epithelial cells. The main finding of this study is that normal epithelial cells from the head and neck region have a much higher tolerance for cisplatin than their neoplastic counterparts. Interestingly, this characteristic is without consequence for growth inhibition. Copyright (C) 1999 Elsevier Science Ireland Ltd.

KW - Cisplatin

KW - Head and neck

KW - Keratinocytes

KW - Non-malignant cells

KW - Pt accumulation

KW - Squamous cells

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Effect of cisplatin exposure on platinum accumulation and growth inhibition in human neoplastic and normal squamous epithelial cells of the mucosa of the upper-aerodigestive tract

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Keywords

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