Effect of IC14, an anti-CD14 antibody, on plasma and cell-associated chemokines during human endotoxemia

Dariusz P. Olszyna; Annelies Verbon; John P. Pribble; Terence Turner; Tim Axtelle; Sander J. H. van Deventer; Tom van der Poll

Effect of IC14, an anti-CD14 antibody, on plasma and cell-associated chemokines during human endotoxemia

Dariusz P. Olszyna, Annelies Verbon, John P. Pribble, Terence Turner, Tim Axtelle, Sander J. H. van Deventer, Tom van der Poll

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

To determine the role of CD14 in lipopolysaccharide (LPS)-induced release of chemokines, 16 humans were injected with LPS (4 ng/kg) preceded (-2 h) by intravenous IC14, an anti-human CD14 monoclonal antibody, or placebo. LPS elicited increases in interleukin (IL)-8 concentrations in plasma and in lysates; of red blood cell (RBC), polymorphonuclear cell and mononuclear cell fractions, which were all reduced by IC14. LPS also induced rises in the plasma and RBC levels of monocyte chemoattractant protein (MCP)-1, which were diminished by IC14. Macrophage inflammatory protein (MIP)-1alpha and MIP-1beta, chemokines that in contrast to IL-8 and MCP-1 can not bind to the Duffy antigen receptor for chemokines on RBCs, were only detected in plasma. IC14 attenuated the LPS-induced release of MIP-1beta, but not of MIP-1alpha. IL-8 and MCP-1, but not MIP-1alpha and MIP-1beta, circulate in RBC-associated form during endotoxemia. LPS-induced chemokine release is, in part, mediated by an interaction with CD14

Original language	English
Pages (from-to)	158-162
Journal	European Cytokine Network
Volume	14
Issue number	3
Publication status	Published - 2003

Cite this

@article{8c2f6fcd699244ab95ca4de84ae713db,

title = "Effect of IC14, an anti-CD14 antibody, on plasma and cell-associated chemokines during human endotoxemia",

abstract = "To determine the role of CD14 in lipopolysaccharide (LPS)-induced release of chemokines, 16 humans were injected with LPS (4 ng/kg) preceded (-2 h) by intravenous IC14, an anti-human CD14 monoclonal antibody, or placebo. LPS elicited increases in interleukin (IL)-8 concentrations in plasma and in lysates; of red blood cell (RBC), polymorphonuclear cell and mononuclear cell fractions, which were all reduced by IC14. LPS also induced rises in the plasma and RBC levels of monocyte chemoattractant protein (MCP)-1, which were diminished by IC14. Macrophage inflammatory protein (MIP)-1alpha and MIP-1beta, chemokines that in contrast to IL-8 and MCP-1 can not bind to the Duffy antigen receptor for chemokines on RBCs, were only detected in plasma. IC14 attenuated the LPS-induced release of MIP-1beta, but not of MIP-1alpha. IL-8 and MCP-1, but not MIP-1alpha and MIP-1beta, circulate in RBC-associated form during endotoxemia. LPS-induced chemokine release is, in part, mediated by an interaction with CD14",

author = "Olszyna, {Dariusz P.} and Annelies Verbon and Pribble, {John P.} and Terence Turner and Tim Axtelle and {van Deventer}, {Sander J. H.} and {van der Poll}, Tom",

year = "2003",

language = "English",

volume = "14",

pages = "158--162",

journal = "European Cytokine Network",

issn = "1148-5493",

publisher = "John Libbey Eurotext",

number = "3",

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TY - JOUR

T1 - Effect of IC14, an anti-CD14 antibody, on plasma and cell-associated chemokines during human endotoxemia

AU - Olszyna, Dariusz P.

AU - Verbon, Annelies

AU - Pribble, John P.

AU - Turner, Terence

AU - Axtelle, Tim

AU - van Deventer, Sander J. H.

AU - van der Poll, Tom

PY - 2003

Y1 - 2003

N2 - To determine the role of CD14 in lipopolysaccharide (LPS)-induced release of chemokines, 16 humans were injected with LPS (4 ng/kg) preceded (-2 h) by intravenous IC14, an anti-human CD14 monoclonal antibody, or placebo. LPS elicited increases in interleukin (IL)-8 concentrations in plasma and in lysates; of red blood cell (RBC), polymorphonuclear cell and mononuclear cell fractions, which were all reduced by IC14. LPS also induced rises in the plasma and RBC levels of monocyte chemoattractant protein (MCP)-1, which were diminished by IC14. Macrophage inflammatory protein (MIP)-1alpha and MIP-1beta, chemokines that in contrast to IL-8 and MCP-1 can not bind to the Duffy antigen receptor for chemokines on RBCs, were only detected in plasma. IC14 attenuated the LPS-induced release of MIP-1beta, but not of MIP-1alpha. IL-8 and MCP-1, but not MIP-1alpha and MIP-1beta, circulate in RBC-associated form during endotoxemia. LPS-induced chemokine release is, in part, mediated by an interaction with CD14

AB - To determine the role of CD14 in lipopolysaccharide (LPS)-induced release of chemokines, 16 humans were injected with LPS (4 ng/kg) preceded (-2 h) by intravenous IC14, an anti-human CD14 monoclonal antibody, or placebo. LPS elicited increases in interleukin (IL)-8 concentrations in plasma and in lysates; of red blood cell (RBC), polymorphonuclear cell and mononuclear cell fractions, which were all reduced by IC14. LPS also induced rises in the plasma and RBC levels of monocyte chemoattractant protein (MCP)-1, which were diminished by IC14. Macrophage inflammatory protein (MIP)-1alpha and MIP-1beta, chemokines that in contrast to IL-8 and MCP-1 can not bind to the Duffy antigen receptor for chemokines on RBCs, were only detected in plasma. IC14 attenuated the LPS-induced release of MIP-1beta, but not of MIP-1alpha. IL-8 and MCP-1, but not MIP-1alpha and MIP-1beta, circulate in RBC-associated form during endotoxemia. LPS-induced chemokine release is, in part, mediated by an interaction with CD14

M3 - Article

C2 - 14656690

SN - 1148-5493

VL - 14

SP - 158

EP - 162

JO - European Cytokine Network

JF - European Cytokine Network

IS - 3

ER -