Effects of fatty acid amide hydrolase inhibition on neuronal responses to nicotine, cocaine and morphine in the nucleus accumbens shell and ventral tegmental area: involvement of PPAR-alpha nuclear receptors

Antonio Luchicchi, Salvatore Lecca, Stefano Carta, Giuliano Pillolla, Anna L Muntoni, Sevil Yasar, Steven R Goldberg, Marco Pistis

Research output: Contribution to journalArticleAcademicpeer-review

76 Citations (Scopus)

Abstract

The endocannabinoid system regulates neurotransmission in brain regions relevant to neurobiological and behavioral actions of addicting drugs. We recently demonstrated that inhibition by URB597 of fatty acid amide hydrolase (FAAH), the main enzyme that degrades the endogenous cannabinoid N-acylethanolamine (NAE) anandamide and the endogenous non-cannabinoid NAEs oleoylethanolamide and palmitoylethanolamide, blocks nicotine-induced excitation of ventral tegmental area (VTA) dopamine (DA) neurons and DA release in the shell of the nucleus accumbens (ShNAc), as well as nicotine-induced drug self-administration, conditioned place preference and relapse in rats. Here, we studied whether effects of FAAH inhibition on nicotine-induced changes in activity of VTA DA neurons were specific for nicotine or extended to two drugs of abuse acting through different mechanisms, cocaine and morphine. We also evaluated whether FAAH inhibition affects nicotine-, cocaine- or morphine-induced actions in the ShNAc. Experiments involved single-unit electrophysiological recordings from DA neurons in the VTA and medium spiny neurons in the ShNAc in anesthetized rats. We found that URB597 blocked effects of nicotine and cocaine in the ShNAc through activation of both surface cannabinoid CB1-receptors and alpha-type peroxisome proliferator-activated nuclear receptor. URB597 did not alter the effects of either cocaine or morphine on VTA DA neurons. These results show that the blockade of nicotine-induced excitation of VTA DA neurons, which we previously described, is selective for nicotine and indicate novel mechanisms recruited to regulate the effects of addicting drugs within the ShNAc of the brain reward system.

Original languageEnglish
Pages (from-to)277-88
Number of pages12
JournalAddiction Biology
Volume15
Issue number3
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Keywords

  • Amidohydrolases
  • Animals
  • Benzamides
  • Carbamates
  • Cocaine
  • Dopamine
  • Evoked Potentials
  • Journal Article
  • Male
  • Morphine
  • Neurons
  • Nicotine
  • Nucleus Accumbens
  • PPAR alpha
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cytoplasmic and Nuclear
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Reward
  • Ventral Tegmental Area

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