TY - JOUR
T1 - Effects of statins on liver cell function and inflammation in septic rats
AU - Stolf, Aline Maria
AU - Lívero, Francislaine dos Reis
AU - Dreifuss, Arturo Alejandro
AU - Bastos-Pereira, Amanda Leite
AU - Fabosi, Isabella Aviles
AU - Alves de Souza, Carlos Eduardo
AU - Gomes, Liana de Oliveira
AU - Chicorski, Raphaella
AU - Brandt, Anna Paula
AU - Cadena, Silvia Maria Suter
AU - Telles, José Ederaldo Queiroz
AU - Hauser, Aline Borsato
AU - Elferink, Ronald Oude
AU - Zampronio, Aleksander Roberto
AU - Acco, Alexandra
PY - 2012
Y1 - 2012
N2 - Background: Several studies suggest that the presence of statins may be beneficial during sepsis, but this idea is controversial. The aim of this study was to investigate the effects of long-term statin treatment in the livers of septic animals, focusing on its antioxidant, antiinflammatory, and metabolic properties. Materials and methods: Male Wistar rats were treated orally with simvastatin, atorvastatin, or vehicle once a d. After 30 d, sepsis was induced by cecal ligation and puncture (CLP) in Control, Simvastatin-treated, and Atorvastatin-treated groups, while the Sham group underwent only laparotomy. The Basal Simvastatin and Basal Atorvastatin groups received only their respective drugs without surgery. Twenty-four h after CLP or laparotomy, samples were collected from anesthetized rats for evaluation of hepatic oxidative stress, liver histology, hepatic mitochondria enzyme activity, leukocyte counts in blood and peritoneal cavity, gene expression of hepatic superoxide dismutase and TNF-2, and plasma biochemistry. Results: Most parameters that we tested exhibited expected changes upon sepsis induction. However, statin treatment only improved liver mitochondrial enzymatic activity. In other parameters, simvastatin and atorvastatin failed to protect the liver against injuries incurred upon the CLP-induced polymicrobial sepsis model. Conclusions: Pretreatment with simvastatin or atorvastatin alone before sepsis induction improved mitochondrial activity in the liver; however, this result was not reproduced in other biomarkers of liver function and leukocyte migration during sepsis. Future studies should be performed to evaluate whether statins can be combined with other drugs to increase the efficacy of sepsis therapy. (C) 2012 Elsevier Inc. All rights reserved
AB - Background: Several studies suggest that the presence of statins may be beneficial during sepsis, but this idea is controversial. The aim of this study was to investigate the effects of long-term statin treatment in the livers of septic animals, focusing on its antioxidant, antiinflammatory, and metabolic properties. Materials and methods: Male Wistar rats were treated orally with simvastatin, atorvastatin, or vehicle once a d. After 30 d, sepsis was induced by cecal ligation and puncture (CLP) in Control, Simvastatin-treated, and Atorvastatin-treated groups, while the Sham group underwent only laparotomy. The Basal Simvastatin and Basal Atorvastatin groups received only their respective drugs without surgery. Twenty-four h after CLP or laparotomy, samples were collected from anesthetized rats for evaluation of hepatic oxidative stress, liver histology, hepatic mitochondria enzyme activity, leukocyte counts in blood and peritoneal cavity, gene expression of hepatic superoxide dismutase and TNF-2, and plasma biochemistry. Results: Most parameters that we tested exhibited expected changes upon sepsis induction. However, statin treatment only improved liver mitochondrial enzymatic activity. In other parameters, simvastatin and atorvastatin failed to protect the liver against injuries incurred upon the CLP-induced polymicrobial sepsis model. Conclusions: Pretreatment with simvastatin or atorvastatin alone before sepsis induction improved mitochondrial activity in the liver; however, this result was not reproduced in other biomarkers of liver function and leukocyte migration during sepsis. Future studies should be performed to evaluate whether statins can be combined with other drugs to increase the efficacy of sepsis therapy. (C) 2012 Elsevier Inc. All rights reserved
U2 - https://doi.org/10.1016/j.jss.2012.08.019
DO - https://doi.org/10.1016/j.jss.2012.08.019
M3 - Article
C2 - 22954522
SN - 0022-4804
VL - 178
SP - 888
EP - 897
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 2
ER -