Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage

Siriwal Suwanpitak, Nutchanawan Promnakhon, Ratchapong Netsrithong, Methichit Wattanapanitch

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

2 Citations (Scopus)


One of the major obstacles for adoptive cell transfer (ACT) of T cells is the loss of effector function and proliferative ability of isolated antigen-specific T cells after prolonged ex vivo expansion. To overcome this issue, induced pluripotent stem cells (iPSCs), which have unlimited proliferation and differentiation potential, can be used to generate a large number of antigen-specific T cells. Here, we describe an efficient differentiation protocol for the generation of cytotoxic CD8+ T cells from human T cell-derived iPSCs (T-iPSCs). The protocol consists of three main steps including differentiation of T-iPSCs toward hematoendothelial progenitors (HEPs), co-culture of HEPs with OP9-DL1 cells, and stimulation of T cell receptor (TCR) signaling to obtain CD8 single-positive (SP) T cells. This culture system is simple and efficient; therefore, will offer a powerful tool for studying T cell development and applications in adoptive immunotherapy.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages20
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology


  • Adoptive cell transfer
  • CD8 T cells
  • Hematoendothelial progenitors
  • Induced pluripotent stem cells
  • OP9-DL1 cells

Cite this