TY - CHAP
T1 - Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage
AU - Suwanpitak, Siriwal
AU - Promnakhon, Nutchanawan
AU - Netsrithong, Ratchapong
AU - Wattanapanitch, Methichit
N1 - Funding Information: This work was supported by Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University (grant number (IO) R016234002 and R016333015), the Thailand Research Fund (grant no. RSA6280090), the Program Management Unit for Human Resources & Institutional Development, Research and Innovation (grant number B05F630080). NP is supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program and Faculty of Medicine Siriraj Hospital, Mahidol University (grant no. PHD/0203/2556). RN is supported by the Development and Promotion of Science and Technology Talents Project. MW is supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. Publisher Copyright: © 2021, Springer Science+Business Media, LLC.
PY - 2022
Y1 - 2022
N2 - One of the major obstacles for adoptive cell transfer (ACT) of T cells is the loss of effector function and proliferative ability of isolated antigen-specific T cells after prolonged ex vivo expansion. To overcome this issue, induced pluripotent stem cells (iPSCs), which have unlimited proliferation and differentiation potential, can be used to generate a large number of antigen-specific T cells. Here, we describe an efficient differentiation protocol for the generation of cytotoxic CD8+ T cells from human T cell-derived iPSCs (T-iPSCs). The protocol consists of three main steps including differentiation of T-iPSCs toward hematoendothelial progenitors (HEPs), co-culture of HEPs with OP9-DL1 cells, and stimulation of T cell receptor (TCR) signaling to obtain CD8 single-positive (SP) T cells. This culture system is simple and efficient; therefore, will offer a powerful tool for studying T cell development and applications in adoptive immunotherapy.
AB - One of the major obstacles for adoptive cell transfer (ACT) of T cells is the loss of effector function and proliferative ability of isolated antigen-specific T cells after prolonged ex vivo expansion. To overcome this issue, induced pluripotent stem cells (iPSCs), which have unlimited proliferation and differentiation potential, can be used to generate a large number of antigen-specific T cells. Here, we describe an efficient differentiation protocol for the generation of cytotoxic CD8+ T cells from human T cell-derived iPSCs (T-iPSCs). The protocol consists of three main steps including differentiation of T-iPSCs toward hematoendothelial progenitors (HEPs), co-culture of HEPs with OP9-DL1 cells, and stimulation of T cell receptor (TCR) signaling to obtain CD8 single-positive (SP) T cells. This culture system is simple and efficient; therefore, will offer a powerful tool for studying T cell development and applications in adoptive immunotherapy.
KW - Adoptive cell transfer
KW - CD8 T cells
KW - Hematoendothelial progenitors
KW - Induced pluripotent stem cells
KW - OP9-DL1 cells
UR - http://www.scopus.com/inward/record.url?scp=85132455752&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/7651_2021_355
DO - https://doi.org/10.1007/7651_2021_355
M3 - Chapter
C2 - 33755900
VL - 2454
T3 - Methods in Molecular Biology
SP - 423
EP - 442
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -