TY - JOUR
T1 - Enolase is regulated by Liver X Receptors
AU - de Boussac, Hugues
AU - Maqdasy, Salwan
AU - Trousson, Amalia
AU - Zelcer, Noam
AU - Volle, David H.
AU - Lobaccaro, Jean-Marc A.
AU - Baron, Silvère
PY - 2015
Y1 - 2015
N2 - Enolase is a glycolytic enzyme known to inhibit cholesteryl ester hydrolases (CEHs). Cholesteryl ester loading of macrophages, as occurs during atherosclerosis, is accompanied by increased Enolase protein and activity. Here, we describe that J774 macrophages treated with LXR agonists exhibit reduced Enolase transcript and protein abundance. Moreover, we show that this reduction is further potentiated by activation of the LXR/RXR heterodimer with the RXR ligand 9-cis retinoic acid. Enolase levels are also reduced in vivo following activation of LXRs in the intestine, but not in the liver. This effect is lost in Lxrαβ-/- mice. In aggregate, our study identified Enolase as a new target of LXRs in vivo, which may promote cholesterol mobilization for subsequent efflux
AB - Enolase is a glycolytic enzyme known to inhibit cholesteryl ester hydrolases (CEHs). Cholesteryl ester loading of macrophages, as occurs during atherosclerosis, is accompanied by increased Enolase protein and activity. Here, we describe that J774 macrophages treated with LXR agonists exhibit reduced Enolase transcript and protein abundance. Moreover, we show that this reduction is further potentiated by activation of the LXR/RXR heterodimer with the RXR ligand 9-cis retinoic acid. Enolase levels are also reduced in vivo following activation of LXRs in the intestine, but not in the liver. This effect is lost in Lxrαβ-/- mice. In aggregate, our study identified Enolase as a new target of LXRs in vivo, which may promote cholesterol mobilization for subsequent efflux
U2 - https://doi.org/10.1016/j.steroids.2015.02.010
DO - https://doi.org/10.1016/j.steroids.2015.02.010
M3 - Article
C2 - 25708389
SN - 0039-128X
VL - 99
SP - 266
EP - 271
JO - Steroids
JF - Steroids
IS - Part B
ER -